NARMS Retail Food Surveillance and Outbreak Investigation in Ohio

项目摘要 为了帮助满足NARMS零售肉类监测项目的需要，作为监测点， 我们将从随机生成的杂货店列表中抽样零售家禽、牛肉和猪肉的包装 周边地区的商店。从80个月的包裹中各取一份进行培养 对于病原体，沙门氏菌和弯曲杆菌，以及两种指示性细菌，大肠杆菌和 肠球菌，以解决可能转移到 病原体。由此产生的分离株将每月运往FDA，以确定物种和血清型。 确认和药敏试验，在我们的 培养收藏。我们预计已成功抽样960包零售牛肉、猪肉和 在每12个月的研究期内，对家禽进行监测。 我们预计，这些努力将有助于提供关键数据，不仅跟踪当前状态 零售业食源性致病菌和指示菌的耐药性分析 肉类，也是为了影响FDA未来的政策和法规，并监测行动的成功 和干预措施。除了我们对零售食品监督计划的贡献外，作为一个 NARMS零售食品参与实验室我们将为食源性疾病提供支持 疫情调查。我们已经与俄亥俄州部门建立了密切的工作关系 卫生实验室和俄亥俄州农业部动物疾病诊断部 实验室。我们将可以直接支持和扩展他们现有的能力 暴发调查和抽样处理和检测。我们还与俄亥俄州立大学密切合作 参与VET-LIRN的兽医中心临床诊断实验室 实验室系统，以及官方发展援助附加系统。我们与这些公司现有的密切合作 位于俄亥俄州中部的实验室将使我们能够有效地作为NARMS零售食品 监督项目实验室，并为支持其他实验室提供支持 相关任务。 我们参与NARMS零售食品监测计划的另一个好处是 我们计划招收多名来自专业兽医和 该项目中的兽医公共卫生项目。对食品安全感兴趣的学生和 抗菌素耐药性和管理将能够获得实验室经验和 了解当前食源性致病菌的抗药性趋势。这些 然后，学生们将离开大学，投身兽医和兽医公共事业 在流行病学、监测战略、食品安全和食品微生物学方面得到更好的培训； 和公共卫生实践。

Project Summary To help address the needs of the NARMS retail meat surveillance project, as a surveillance site, we will sample packages of retail poultry, beef and pork from a randomly generated list of grocery stores in the surrounding area. Aliquots from each of eighty monthly packages will be cultured for pathogens, Salmonella and Campylobacter, along with two indicator bacteria, E. coli and Enterococcus, to address potential resistance mechanisms which may be transferred to pathogens. The resulting isolates will be shipped monthly to FDA for species and serotype confirmation and antimicrobial susceptibility testing, with duplicate isolates maintained in our culture collection. We expect to have successfully sampled 960 packages of retail beef, pork, and poultry during each 12-month study period. We expect that these efforts will help to provide critical data to not only track the current state of antimicrobial resistance among foodborne pathogens and indicator bacteria found in retail meat, but also to impact future FDA policy and regulations and monitor the success of actions and interventions. In addition to our contributions to the retail food surveillance program, as a NARMS retail food participating laboratory we will be available to support foodborne disease outbreak investigation. We already have a close working relationship with the Ohio Department of Health laboratory and the Ohio Department of Agriculture’s Animal Disease Diagnostic Laboratory. We will be available to directly support and extend their existing capability for outbreak investigation and sample process and testing. We also collaborate closely with the OSU Veterinary Medical Center Clinical Diagnostic Laboratory, which participates in the Vet-LIRN laboratory system, as does the ODA ADDL. Our existing close collaborations with these laboratories located in central Ohio will allow us to be effective as a NARMS retail food surveillance project laboratory, and to contribute to the support of other laboratories with related missions. An additional benefit of our participation in the NARMS retail food surveillance program is that we plan to engage multiple students from both the professional veterinary medicine and veterinary public health programs in this project. Students with an interest in food safety and antimicrobial resistance and stewardship will be able to gain laboratory experience and an understanding of the current antimicrobial resistance trends in foodborne pathogens. These students will then leave the university for careers in veterinary medicine and veterinary public health better trained in epidemiology, surveillance strategies, food safety and food microbiology, and public health practice.