Transgenic & Gene-Targeting Shared Resource

转基因和基因靶向共享资源-项目摘要 对于堪萨斯癌症大学的研究人员来说，基因改变的小鼠模型是重要的工具 中心（KUCC）。这些模型的制作和分析最终会使人们更好地理解 肿瘤形成的性质、进展和功能基因组学。它们也可作为体内模型， 诊断和治疗。转基因和基因靶向共享资源（TGTSR），领导杰伊L。薇薇安 博士，支持KUCC的成员提供集中和全面的技术服务， 生产新型转基因和基因靶向啮齿动物和遗传改变的多能干细胞。的 TGTSR使用尖端的方法，最先进的仪器和新型试剂来产生 这些模型。TGTSR位于KUMC校园内，有四名全职技术人员沿着。 TGTSR的癌症中心支持允许在该设施中制定具体举措 与癌症研究有关。例如，某些转基因方法和突变特别相关， 癌症研究，包括组织特异性转基因表达，临床上重现的细微突变， 鉴定的变体和体细胞突变以及菌株特异性核转移。新兴技术，包括 体内基因组编辑方法（例如CRISPR/Cas9和TALE核酸酶）提供了更快速的手段， 产生这些类型的肿瘤进展的新型小鼠模型。这些不断的整合 将方法演变成TGTSR的“工具箱”，大大加速了动物模型的发展。 癌症，同时也降低了所有校园的KUCC研究人员的成本。

Transgenic and Gene-Targeting Shared Resource – Project Summary Genetically altered mouse models are important tools for the researchers at the University of Kansas Cancer Center (KUCC). The production and analysis of such models ultimately leads to a better understanding of the nature, progression and functional genomics of tumor formation. They also serve as in vivo models for diagnostics and treatment. The Transgenic and Gene-Targeting shared resource (TGTSR), led Jay L. Vivian PhD, supports members of KUCC by providing centralized and comprehensive technical services for the production of novel transgenic and gene-targeted rodents and genetically altered pluripotent stem cells. The TGTSR uses cutting edge methods, state-of-the-art instrumentation, and novel reagents for the generation of these models. The TGTSR, housed at the KUMC campus, has four full time technical staff along with Vivian. The Cancer Center support of the TGTSR allows for the development of specific initiatives in the Facility relevant to cancer research. For example, certain transgenic methods and mutations are particularly relevant to cancer studies, including tissue specific transgene expression, subtle mutations that recapitulate clinically identified variants and somatic mutations and strain-specific nuclear transfer. Emerging technologies, including in vivo genome editing methods (e.g. CRISPR/Cas9 and TALE nucleases) are providing a more rapid means of generating these types of novel mouse models of tumor progression. The integration of these continually evolving methods into the `toolbox' of the TGTSR greatly accelerates the development of animal models of cancer, while also reducing costs to KUCC researchers on all campuses.