Core A: Lipidomics

摘要 PPG中的脂质组学核心的目的是提供智力和体力资源(特别是 鞘磷脂和合成分子工具的最新质谱分析)以增强 了解生物活性脂类在癌症生物学中的作用，以期发现未来的治疗方法。这个 生物活性脂质的多样性及其相互关联的新陈代谢产生了一个调节途径的网络 细胞内和细胞间的信号和功能。这些通路的功能障碍导致了脑膜瘤的病理生物学 癌症的进展和转移。这些考虑使得脂质的发展成为必要。 化学和分析。脂肪组学核心是基于以下关键人员的独特专业知识创建的 脂肪化学、分析和新陈代谢。这个核心已经发展成为一个制度性的、全国性的和 脂类组学和脂类化学生物学新兴领域的国际资源。核心服务包括：1) 提供来自不同生物材料(细胞、 组织和生物液体)，主要使用高效液相色谱-串联质谱仪 光谱分析(LC-MS/MS)技术-Core目前提供超过300个 基础代谢水平上不同的脂类分子种类；2)开发和提供合成分子 研究脂质代谢的工具(例如，功能化和荧光神经酰胺、部位特异性放射性 鞘磷脂、17C-鞘磷脂)和用于细胞、体外和体内的各种合成脂质和类似物 研究(例如，细胞器靶向的鞘脂和细胞器靶向的鞘脂代谢抑制剂 酶)；3)提供直接的MALDI-MS组织分析，以可视化基于脂质的抗癌药物的分布 药物及其对鞘磷脂成分的分子作用；以及4)协助PPG调查人员 实验设计、感兴趣的脂类的选择和分析结果的解释。

SUMMARY The purpose of the Lipidomics Core in the PPG is to provide intellectual and physical resources (particularly state-of-the-art mass spectrometry analysis of sphingolipids and synthetic molecular tools) to enhance understanding of the role of bioactive lipids in cancer biology with the goal to discover future therapeutics. The diversity of bioactive lipids and their interconnected metabolism generates a network of pathways regulating intra- and inter-cellular signaling and function. Dysfunctions in these pathways contribute to the pathobiology of cancer progression and metastasis. These considerations have necessitated the development of lipid chemistry and analysis. The Lipidomics Core was created based on unique expertise of the key personnel in lipid chemistry, analysis, and metabolism. This Core has developed into an institutional, national, and international resource in the emerging fields of lipidomics and lipid chemical biology. Core services include: 1) Providing qualitative and quantitative analysis of lipid components from different biological materials (cells, tissue, and biological fluids), primarily employing high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) technology—the Core currently provides quantitative analysis of more than 300 distinct lipid molecular species at a basic metabolomic level; 2) Developing and providing synthetic molecular tools to study lipid metabolism (e.g., functionalized and fluorescent ceramides, site-specific radioactive sphingolipids, 17C-sphingolipids) and diversified synthetic lipids and analogs for cellular, in vitro, and in vivo studies (e.g., organelle-targeted sphingolipids and organelle-targeted inhibitors of sphingolipid metabolizing enzymes); 3) Providing direct MALDI-MS tissue analysis to visualize the distribution of lipid based anti-cancer drugs and their molecular effects on sphingolipid components; and 4) Assisting PPG investigators in experimental design, selection of lipids of interest, and interpretation of analytical results.