Core C

项目概要/摘要-核心C 体外电生理核心（核心C）将为体外 电生理学研究使用新的工具，如光遗传学，设计受体 专门由设计药物（DREADD）和体外GCaMP 6钙激活 显像我们将准备大脑切片，进行膜片钳记录，优化 用于光遗传学和化学遗传学实验的方案，进行Ca 2+成像 研究在体外切片，并进行通道视紫红质2（ChR 2）辅助电路 映射（CRACM）。Core还将对记录的切片进行组织学检查， 分析相关数据。项目1（Saper）、项目2（Scammell）、项目3（Fuller） 项目4（Vanderhost和Dymecki）将使用该核心设施。这些项目 未配备进行体外电生理学记录的设备， 可以提供这种服务的机构资源。这些项目将受益于 核心的专业知识和共享资源。

Project Summary/Abstract- Core C The in vitro Electrophysiology Core (Core C) will provide services for in vitro electrophysiological studies using novel tools such as optogenetics, designer receptors exclusively activated by designer drugs (DREADDs) and in vitro GCaMP6 calcium imaging. We will prepare brain slices, conduct patch-clamp recordings, optimize the protocols for the optogenetic and chemogenetic experiments, perform Ca2+ imaging studies in in vitro slices, and conduct channelrhodopsin2 (ChR2)-assisted circuit mapping (CRACM). The Core will also perform histology of the recorded slices, and analyze the associated data. Project 1 (Saper), Project 2 (Scammell), Project 3 (Fuller) and Project 4 (Vanderhost and Dymecki) will use this Core facility. These projects are not equipped to conduct in vitro electrophysiology recordings and there are no institutional resources that can provide this service. The Projects will benefit from the expertise and shared resources of the Core.