Defining Brainstem Neuronal Pathways for Orofacial Motor Actions

项目1。摘要 定义口面运动动作的脑干神经元通路 本研究项目将定义神经元表型及其连接性，即，“组成部分” 和“接线图”，用于电机前和更高阶，例如，节律性口面运动的前2运动通路 行动这将揭示协调电机动作的控制结构。这项工作的主要技术 是新生和成年小鼠的跨突触病毒示踪，交叉标记策略， 成年动物的跨突触追踪，以及操纵神经元活动的交叉策略， 推断功能。成人数据将被添加到可训练的基于纹理的数字地图集（项目5和核心2） 这些数据将产生必要的电路orofacial探索行动。我们的五年展望是解剖学的 识别关键口面感觉的前运动通路和前2运动通路： 由鼻子，位移的位置mystyrene垫驱动设定点的变化，并完成电路 用来搅拌和摇头。我们进一步考虑了运动控制的解剖电路， 舌头用于搜索或辨别任务中的运动动作，沿着发声。 这些数据提供了一组连接，这些连接限制了口面神经激活和操作的模型。 探索性电路它们让我们深入了解自然界在网络设计中所做的操作权衡。 这些数据，沿着来自研究项目2和3的互补解剖学和电生理学数据， 为协调不同口面动作的控制机制的理论分析提供输入 （4）勘探项目。

Project 1. Abstract Defining brainstem neuronal pathways for orofacial motor actions This Research Project will define the neuronal phenotypes and their connectivity, i.e., the “Components" and "Wiring diagrams", for premotor and higher order, e.g., pre2motor pathways for rhythmic orofacial motor actions. This will reveal the control structure for coordinating motor actions. Primary technologies for this work are transsynaptic viral tracing in both neonates and adult mice, intersectional labeling strategies followed by transsynaptic tracing in adult animals, and intersectional strategies to manipulate neuronal activity in order to infer function. Adult data will be added to the Trainable Texture-based Digital Atlas (Project 5 and Core 2) These data will yield essential circuitry for orofacial exploratory actions. Our five-year horizon is anatomical identification of pre- and pre2motor pathways for key orofacial senses: twitching and directional movement of by the nose, shifts in the position of the mystacial pad driving set point changes, and completion of the circuit for whisking, bobbing of the head. We further consider anatomical circuitry underlying motor control of the tongue for motor actions involved in search or discrimination tasks, along with vocalization. These data provide a set of connections that constrain models for the activation and operation of orofacial exploratory circuitry. They give insight into the operational tradeoffs that nature has made in network design. These data, along with complementary anatomy and electrophysiology data from Research Projects 2 and 3, provide input for a theoretical analyses of the control mechanisms that coordinate different orofacial actions during exploration (Project 4).