Cooperative Human Tissue Network Support through Duke's BioRepository & Precision Pathology Center

项目摘要 高质量的生物样品对癌症研究至关重要。该项目利用经CAP认证的 杜克大学生物库的基础设施、人力资源、经验和设备 精准病理中心(BRPC)主任、团队成员及其附属网络核心 通过国家癌症研究所的实验室为研究人员提供高质量的生物标本 合作人体组织网络(CHTN)。这和CHTN额外的生物库BEST任务 通过以下四个具体目标开展实践传播和社区教育。 目的1：高效地为CHTN研究人员提供高质量的组织、血液和液体生物检验品 基础和早期翻译研究。标本采集通过BRPC及其网络提供便利 最佳实践生物库，包括加州大学洛杉矶分校的生物库 密歇根大学、圣路易斯华盛顿大学、北卡罗来纳大学教堂山分校、 和芝加哥大学。在杜克大学和网络地点使用广泛同意可以提供 在长期临床注释方面具有最大的灵活性。样品加工中的最新技术 包括活体标本保存、通过宏观或微观解剖进行肿瘤浓缩、核酸 提取，基因分型，生物标记物表型，全玻片成像，组织微阵列， 免疫组织化学和原位杂交，以及数字图像分析也是可用的。目标2： 提供咨询。杜克大学病理学家和生物库工作人员为CHTN提供专家咨询 研究人员对人类生物样本的使用、检测选择和样品处理进行了研究，并着眼于 适合用途的预先分析。目标3：开发和传播关于高质量做法的知识 生物仓库。CHTN团队将承担新的项目，并通过出版发布成果 以及在国家和国际会议上的介绍。PI将继续在国家和地区服务 生物库社区内的国际领导角色。目标4：教育当地、地区和 关于生物标本在生物医学研究中的重要性的国家社区。在当地，杜克大学团队 将扩大社区“生物库之旅”，教育北卡罗来纳州达勒姆的居民 包括研究中的组织和血液样本，特别是增加样本数量 来自代表人数不足的少数族裔。在全国范围内，PI将继续寻找机会 教育公众和科学界，让他们知道拥有足够、高质量、 可供研究的带注释的生物标本。例如全国性的播客和会议 面向普通科学研究受众的演示文稿，并利用印刷、音频 以及面向大众的视频新闻，如时事通讯、社交媒体帖子和 采访。

PROJECT ABSTRACT High-quality biospecimens are critical to cancer research. This project leverages the CAP-accredited biorepository infrastructure, human resources, experience and equipment of the Duke BioRepository & Precision Pathology Center (BRPC) director, team members and its' affiliated networked core laboratories to provide high-quality biospecimens to investigators through the National Cancer Institute's Cooperative Human Tissue Network (CHTN). This and the additional CHTN missions of biobanking best practice dissemination and community education are addressed through the following four specific aims. Aim 1: Efficiently provide high-quality tissue, blood and fluid biospecimens to CHTN investigators for basic and early translational research. Specimen collection is facilitated through BRPC and its network of best practice biorepositories including those at the University of California at Los Angeles, the University of Michigan, Washington University at St. Louis, University of North Carolina at Chapel Hill, and the University of Chicago. The use of broad consent at Duke and in network locations provides maximum flexibility in long-term clinical annotation. The latest technologies in sample processing including viable specimen preservation, tumor enrichment by macro or microdissection, nucleic acid extraction, genotyping, biomarker phenotyping, whole slide imaging, tissue microarray, immunohistochemistry and in situ hybridization, and digital image analysis are also available. Aim 2: Provide consultation. Duke pathologists and biorepository staff provide expert consultation to CHTN investigators on use of human biospecimens, assay selection and sample processing with an eye toward fit-for-purpose pre-analytics. Aim 3: Develop and disseminate knowledge on high-quality practices for biorepositories. This CHTN team will take on new projects and disseminate results through publication and presentation at national and international meetings. The PI will continue to serve in national and international leadership roles within the biorepository community. Aim 4: Educate the local, regional and national community on the importance of biospecimens in biomedical research. Locally, the Duke team will expand community `biobank tours' to educate the Durham, NC population on the importance of including tissue and blood samples in research and specifically increasing the number of samples available from underrepresented minorities. Nationally, the PI will continue to seek opportunities to educate the public and the scientific community about the importance of having sufficient, high-quality, annotated biospecimens available for research. Examples include national podcasts and meeting presentations geared toward general scientific research audiences and taking advantage of print, audio and video journalism geared toward the lay public such as newsletters, social media postings and interviews.