Proteomics Shared Resource

蛋白质组学共享资源 导演：拉里·大卫，博士。 摘要/项目摘要 奈特癌症研究所(Knight)赞助的蛋白质组学共享资源提供了先进的工具和 具有确定蛋白质的特性、丰度和修饰的质谱学专业知识。 这些分析对于了解癌症的原因和设计改进的治疗方法是至关重要的 疾病。该资源支持广泛的定量测量，包括：检测差异 来源于原发肿瘤、细胞系、培养的肿瘤器官和 患者来源的异种移植样本；翻译后修饰的检测，如磷酸化， 乙酰化、泛素化、甲基化和O-GlycN酰化，测定新合成的蛋白质； 蛋白质/蛋白质相互作用的研究；以及蛋白质结构的表征。该设施是一所大学- 广泛的资源，利用该机构强有力的财政和行政支持来加强癌症 在协作环境中跨校园和姊妹机构进行研究。它雇佣了六名训练有素的员工 个人，五个博士水平，维护着四个质谱仪系统，其中一个是最先进的 高分辨率轨道诱饵聚变仪。其中两名工作人员提供全职信息学支持，这有一个 在该设施的成功中发挥着关键作用。此外，蛋白质组学共享资源受益于参与 OHSU太平洋西北地区综合有机体国家实验室，提供全面的专业知识 和仪器，可通过太平洋蛋白质组学的NIGMS生物技术研究资源获得 西北国家实验室。来自CCSG赠款的资金为设施主任提供10%的工资支持， 副主任10%的工资支持。蛋白质组学共享资源将继续与 骑士和其他OHSU利益相关者识别和实施符合新技术和测试的新技术和测试 研究机会和不断发展的需求，包括与骑士密切合作，提供工具和 新骑士早期发现计划所需的支持。

PROTEOMICS SHARED RESOURCE Director: Larry David, Ph.D. ABSTRACT/PROJECT SUMMARY The Knight Cancer Institute (Knight) sponsored Proteomics Shared Resource provides advanced tools and expertise in mass spectrometry to determine the identity, abundance, and modifications occurring in proteins. These analyses are crucial to understanding the cause of cancer and devising improved treatments for the disease. The resource supports a wide array of quantitative measurements including: detection of differential protein abundance in complex cell lysates derived from primary tumors, cell lines, cultured tumor organoids and patient-derived xenograft samples; detection of post-translation modifications, such as phosphorylation, acetylation, ubiquitination, methylation and O-GlycNAcylation, measurement of newly synthesized proteins; examination of protein/protein interactions; and, characterization of protein structure. The facility is a university- wide resource that leverages strong financial and administrative support from the institution to enhance cancer research across campus and sister institutions in a collaborative environment. It employs six highly trained individuals, five at the Ph.D. level, and maintains four mass spectrometer systems, one that is a state-of-the-art high-resolution Orbitrap Fusion instrument. Two of the staff provide full-time informatics support, which has a key role in the success of the facility. In addition, the Proteomics Shared Resource benefits from participation in the OHSU Pacific Northwest National Laboratory for Integrated Omics, which provides full access to expertise and instrumentation available through the NIGMS BioTechnology Research Resource in Proteomics at Pacific Northwest National Laboratory. Funds from this CCSG grant provide 10% salary support for the facility director, and 10% salary support for the associate director. The Proteomics Shared Resource will continue to work with the Knight and other OHSU stakeholders to identify and implement new technologies and assays that meet new research opportunities and evolving needs, including close collaboration with the Knight in providing tools and support needed by the new Knight early detection initiative.