Musculoskeletal Structure and Strength Core

摘要-资源核心B 在评估动物模型的功能结果时，结构和强度的测量是必不可少的 与肌肉骨骼生物学和医学相关。评估这些结果需要专门的 设备和专业知识，在多个实验室中重建是不切实际和低效的。作为对此的回应 需要时，我们建立了肌肉骨骼结构和力量核心。这个多方面的资源支持 我们的研究社区了解和实施评估结构和 源自的肌肉骨骼组织(骨、软骨、椎间盘、肌肉、肌腱)的力学特性 动物模型。我们已经产生了很大的影响。自2009年以来，我们已为94个独特的客户提供收费服务 调查人员报告了176篇被引用3266篇手稿的结构或强度数据 泰晤士报。对这些服务的需求仍然很高，目前和预计未来将有57个用户。为了 在未来，我们提出了两个具体目标，以维持和扩展这一资源。目标1-成像：维护 方案和设备，提供培训和技术支持，并进行肌肉骨骼成像 由研究界研究人员产生的动物模型的结构和组织。例程 技术包括平面射线照相术、双能X射线吸收法(DXA)和微型计算机 体层摄影术(MicroCT)，每一种都可以在死后或活体内进行。我们最近开发了一些方法来 对比度增强成像；我们将向用户提供这些功能，并将继续开发新的 非矿化组织(软骨、椎间盘、肌肉、肌腱)的成像方法。我们将定期为您呈现 举办成像研讨会，并加强我们与细胞成像中心的伙伴关系。目标2- 机械测试：进行机械测试以评估肌肉骨骼的功能特性 由研究界研究人员产生的动物模型的结构和组织。标准 测试方法包括长骨弯曲、椎体压缩、肌腱和肌腱对骨的拉伸。 测试，和骨微压痕。新建立的方法包括被动和主动肌肉张力。 断裂韧性的测试和缺口弯曲。我们将举办研讨会并提供培训和 丰富了中心研究人员对生物力学的理解。总而言之，核心B 将为我们大型研究社区的成员提供关键支持，以实现经济高效的利用 量化肌肉骨骼组织(骨、肌腱、 肌肉、软骨、盘)。通过我们的努力，我们将提高研究效率和严谨性，并提供 获得最先进和新兴的技术。

Summary – Resource Core B Measures of structure and strength are essential when assessing functional outcomes in animal models related to musculoskeletal biology and medicine. Evaluating these outcomes requires specialized equipment and expertise, which is impractical and inefficient to recreate in multiple labs. In response to this need, we established the Musculoskeletal Structure and Strength Core. This multifaceted resource supports our Research Community in understanding and implementing techniques to evaluate structure and mechanical properties of musculoskeletal tissues (bone, cartilage, disc, muscle, tendon) derived from animal models. We have had a strong impact. Since 2009, we have provided billable services to 94 unique investigators, who have reported structure or strength data in 176 manuscripts which have been cited 3266 times. The need for these services remains high, with 57 current and projected future users. In order to sustain and extend this resource going forward, we propose two Specific Aims. Aim 1 – Imaging: Maintain protocols and equipment, provide training and technical support, and perform imaging of musculoskeletal structures and tissues from animal models generated by Research Community investigators. Routine techniques include plane radiography, dual-energy X-ray absorptiometry (DXA), and micro-computed tomography (microCT), each available post mortem or in vivo. We have recently developed methods for contrast-enhanced imaging; we will make these available to our users and will continue to develop new approaches for imaging non-mineralized tissues (cartilage, disc, muscle, tendon). We will present regular seminars on imaging and strengthen our partnership with the Center for Cellular Imaging. Aim 2 – Mechanical Testing: Perform mechanical testing to assess the functional properties of musculoskeletal structures and tissues from animal models generated by Research Community investigators. Standard testing methods include long-bone bending, vertebral compression, tendon and tendon-to-bone tensile testing, and bone microindentation. Newly established methods include passive and active muscle tension testing and notched bending for fracture toughness. We will present seminars and provide training and enrichment to enhance understanding of biomechanics among Center investigators. In summary, Core B will provide critical support to members of our large Research Community to enable cost-effective utilization of methods to quantify morphology and mechanical properties from musculoskeletal tissues (bone, tendon, muscle, cartilage, disc). Through our efforts we will enhance research productivity and rigor, and provide access to state-of-the-art and emerging techniques.