MHC Genetic Typing

项目摘要/摘要： 该应用程序的主要目标是在加勒比海地区维持无特定病原体(SPF)计划 灵长类动物研究中心(CPRC)通过运营、管理、兽医护理、病毒学支持 和MHC分型，以及我们全面遗传管理计划的改进。的一个独特功能 CPRC动物在支持SPF殖民地的成功建立和可持续方面是这些动物 自然不含SIV和SRV-D，这是四种待检测病毒中的两种。尽管如此，为了安全和按要求 对于SPF计划，我们继续对所有四种病毒(B病毒、STLV、SIV和SRV-D)进行系统测试。这个 该计划为纯印度血统的恒河猴提供明确的遗传和病毒学背景。 群体遗传分析表明，SPF群体随着时间的推移保持了遗传同质性 具有足够的杂合度和来自其非SPF创建者的最小分层。遗传 将使用本文件中描述的遗传管理策略在SPF群体中保持变异 申请。基因管理方法将允许在需求中丰富所需的MHC标记 用于生物医学研究，同时保持遗传多样性。这一特定的核心是为了延续我们目前的 努力保持遗传多样性、MHC定义的SPF印度源恒河猴群体，用于 与艾滋病相关的研究。2015年，KDL开始与目前的U42合作，设计和改进 SPF选育菌落以提高菌落维护效率以及最大化遗传 群体动物的异质性。从那时起，我们一直在构建多代SPF家系 CPRC可以追溯到卡约圣地亚哥岛上的创建者。

PROJECT SUMMARY/ABSTRACT: The major goal of this application is to sustain the Specific Pathogen Free (SPF) program at the Caribbean Primate Research Center's (CPRC) through support of operations, administration, veterinary care, virological and MHC typing and by the improvement of our comprehensive genetic management plan. A unique feature of CPRC animals in favoring a successful establishment and sustainable of SPF colony are that these animals are naturally free of SIV and SRV-D, two of the four viruses to be tested for. Nevertheless, for safety and as required for the SPF Program, we continue systematically testing for all four viruses (B virus, STLV, SIV and SRV-D). The Program provides unadmixed Indian-origin rhesus monkeys with defined genetic and virological background. The population genetic analyses revealed that the SPF colony has remained genetically homogenous over time with sufficient amounts of heterozygosity and minimal stratification from its non-SPF founders. Genetic variation will be maintained in the SPF colony using the genetic management strategies described in this application. The genetic management approach will allow for enrichment of desired MHC markers in demand for biomedical research while maintaining genetic diversity. This specific core is designed to continue our current efforts to maintain a genetically diverse, MHC-defined SPF Indian-origin rhesus macaque colony for use in AIDS-related research. In 2015, the KDL began its collaboration with the current U42 to design and improve the SPF breeding colony to enhance the efficiency of colony maintenance as well as to maximize genetic heterogeneity of the colony animals. Since then we have been constructing multigenerational SPF pedigrees at the CPRC which can be traced back to founders in the Cayo Santiago island population.