Wide-Field Short-Wave Infrared (SWIR) multiphoton (MP) tissue imaging

TRD1：荧光光谱学和显微技术 调查人员：P.So(1.1，1.2)[领先]；M.Bawendi(1.2)；G.Schlau-Cohen(1.3) 合作项目：贾恩(CP1)、博伊登(CP4)、坎帕诺拉(CP8)、科尔曼(CP9) 项目摘要：荧光光谱学和成像技术是 生物医学研究社区。在LBRC，调查人员在精确地利用他们的专业知识 光谱学、造影剂开发和超快脉冲的相干空间和时间控制 开发针对特定分析物的生物系统研究的尖端技术，从蛋白质到 整个有机体。这种基于荧光的TRD建立在3D光雕技术和短波的基础上 本周期开发的红外(SWIR)技术有三个令人振奋的新方向：高通量 深度SWIR成像(TRD1.1)、高通量、超分辨率3D成像(TRD1.2)，以及纳米- 蛋白质运动的尺度研究(TRD1.3)。这些方向是由LBRC合作推动的。被推开 体内肿瘤内肿瘤生物学的研究，特别是对血液等动态事件的监测 血流和氧合变化(CP1)，TRD1.1旨在通过以下方式优化成像速度和深度 图案化双光子时间聚焦广场激发与压缩传感算法相结合 成像超亮量子点(TRD4)。博伊登博士的工作也推动了绘制连接图的工作 大脑(CP4)，这反过来需要以50 nm分辨率高通量识别突触裂隙 在0.5立方米的体积内。基于我们在结构照明(SI)和点扩散函数方面的专业知识 (PSF)工程，TRD1.2寻求将超分辨率成像速度提高到接近1G体素/秒 以便在~1年内绘制出整个大脑的图谱。同样的超分辨率方法也适用于高分辨率的 用于控制癌细胞迁移和组织的细胞外基质的3D微加工 再生(CP8)。最后，由于需要对受体的信号机制有新的了解， 哪些是癌症治疗的目标(CP9)，TRD1.3将开发荧光光谱工具 纳米空间和亚毫秒时间分辨率。总之，这一TRD进一步扩展了核心 通过引入这三个新的研究方向，增强了LBRC在荧光仪器方面的优势。

TRD 1: FLUORESCENCE SPECTROSCOPY AND MICROSCOPY TECHNIQUES Investigators: P. So (1.1, 1.2) [lead]; M. Bawendi (1.2); G. Schlau-cohen (1.3) Collaborative Projects: Jain (CP1), Boyden (CP4), Campagnola (CP8), Coleman (CP9) Project Summary: Fluorescence spectroscopy and imaging are key techniques in the repertoire of the biomedical research community. In the LBRC, the investigators leverage their expertise in precision spectroscopy, contrast agent development, and coherent spatial and temporal control of ultrafast pulses to develop cutting-edge technologies for analyte-specific investigation of biological systems, from proteins to whole organisms. This fluorescence-based TRD builds upon 3D light sculpting techniques and short-wave infrared (SWIR) technologies developed in the current cycle with three exciting new directions: high-throughput deep SWIR imaging (TRD1.1), high-throughput, super-resolution 3D imaging (TRD1.2), and the nanometer- scale study of protein motions (TRD1.3). These directions are motivated by LBRC collaborations. Pushed by the study of cancer biology inside thick solid tumors in vivo, especially for monitoring dynamic events like blood flow and variations in oxygenation (CP1), TRD1.1 seeks to optimize both imaging speed and depth by combining patterned two-photon temporally focused wide-field excitation with compressive-sensing algorithms to image ultra-bright quantum dots (TRD4). Pushed also by Dr. Boyden's work to map the connection diagram of the brain (CP4), which in turn requires high-throughput identification of synaptic clefts at 50 nm resolution throughout a 0.5 cm3 volume. Based on our expertise in structured illumination (SI) and point spread function (PSF) engineering, TRD1.2 seeks to improve super-resolution imaging speed to approach 1G voxel/sec in order to map the whole brain within ~1 year. The same super-resolution approach is employed for high- throughput 3D microfabrication of an extracellular matrix to control cancer cell migration and tissue regeneration (CP8). Finally, pushed by the need for new insight into the signaling mechanisms of receptors, which are the targets of cancer therapeutics (CP9), TRD1.3 will develop fluorescence spectroscopy tools with nanometer spatial and sub-millisecond temporal resolution. In summary, this TRD further extends the core strength of the LBRC in fluorescence instrumentation by introducing these three new research directions.