Cellular Senescence and Beyond Core

摘要 细胞命运是年龄相关分子事件之间的重要中介，包括损伤和 压力，以及作为生物体老化标志的表型和病理。细胞命运也可以提供 独特的--而且广泛适用--干预许多与年龄有关的疾病的机会。这个 此Nathan Shock Center(NSC)应用程序中的细胞衰老和超越核心(CSB)将有所帮助 学术研究人员确定特定的细胞命运是否以及在多大程度上对它们的衰老和/或 干预模式。根据项目的不同，核心将以细胞自治和非 选定细胞命运的自主特征，向研究人员提供其可能的生理后果， 并测试提出的干预策略。目标1将建立并提供标准化方法来检测细胞- 细胞命运的相关标记物决定细胞衰老、细胞死亡和细胞竞争。目标2将 建立标准化方法来检测和评估由此产生的细胞非自治效应的影响 从细胞命运决定细胞衰老、细胞死亡和细胞竞争。核心将采用一种 各种技术，包括ELISA、免疫细胞化学、西方分析以及特别有用的质量 光谱分析，提供全面、公正的评估。核心还将就以下问题向调查人员提供建议 确定最有希望的细胞自主和非自主代理候选人的策略并执行 相关检测以测试这些候选者的生物活性。总而言之，拟议的蜂窝 衰老和超越核心将为衰老研究的研究人员提供广泛的实验 关于如何确定选定的细胞命运决定是否重要以及在多大程度上重要的分析和建议 研究人员感兴趣的年龄相关表型和病理的贡献者。

ABSTRACT Cell fates are important intermediates between age-related molecular events, including damage and stress, and the phenotypes and pathologies that are hallmarks of aging organisms. Cell fates can also offer unique -- and broadly applicable -- opportunities for interventions into many age-related diseases. The Cellular Senescence and Beyond Core (CSB) in this Nathan Shock Center (NSC) application will help academic researchers determine whether and to what extent specific cell fates play a role in their aging and/or intervention models. Depending on the project, the Core will characterize cell autonomous and non- autonomous features of selected cell fates, advise investigators of their possible physiological consequences, and test proposed intervention strategies. Aim 1 will establish and offer standardized methods to detect cell- associated markers of the cell fate decisions of cellular senescence, cell death and cell competition. Aim 2 will establish standardized methods to detect and assess the impact of the cell non-autonomous effects resulting from the cell fate decisions of cellular senescence, cell death and cell competition. The core will employ a variety of techniques, including ELISAs, immunocytochemistry, western analyses and, of particular utility, mass spectrometry to provide comprehensive, unbiased assessments. The Core will also advise investigators on strategies to identify the most promising cell autonomous and non-autonomous acting candidates and perform relevant assays to test these candidates for biological activity. In summary, the proposed Cellular Senescence and Beyond Core will provide investigators in aging research with a broad array of experimental assays and advice on how to determine whether and to what extent selected cell fate decisions are important contributors to the age-related phenotypes and pathologies of interest to the investigators.