Molecular Biology Core

项目总结 所有第二阶段的Cobre项目都利用分子生物学的方法来理解分子 包括流感病毒在内的呼吸道病原体引起的呼吸道疾病的机制(项目1和 项目2)、肺炎链球菌(项目3)和铜绿假单胞菌(项目4)。整体而言 分子生物学核心(MBC)的目标是在以下领域提供集中服务和技术专长 第二阶段科布雷项目调查员、试点项目调查员和其他调查员的分子生物学。 MBC的具体目标是为1)使用RNAi和 CRISPR/Cas9，转基因过表达和突变/缺失，2)全基因组基因图谱 RNA测序，3)分子分析，包括报告分析和实时定量聚合酶链式反应，4)试剂 用于呼吸道和传染病研究，包括原代肺细胞、呼吸道病毒和细菌， 5)对分子生物学各方面的建议、咨询和培训，开发或采用 根据需要采用新的方法。

PROJECT SUMMARY All the Phase II CoBRE projects utilize molecular biology approaches to understand the molecular mechanisms of respiratory diseases caused by respiratory pathogens, including influenza virus (Project 1 and Project 2), Streptococcus pneumoniae (Project 3) and Pseudomonas aeruginosa (Project 4). The overall objective of the Molecular Biology Core (MBC) is to provide centralized services and technical expertise in molecular biology for Phase II CoBRE project investigators, pilot project investigators, and other investigators. The specific aims of the MBC are to provide services and support for 1) functional studies using RNAi and CRISPR/Cas9, transgene overexpression, and mutagenesis/deletion, 2) genome-wide gene profiling using RNA sequencing, 3) molecular assays including reporter assays and real-time quantitative PCR, 4) reagents for respiratory and infectious disease research including primary lung cells, respiratory viruses and bacteria, and 5) advice, consultation and training on various aspects of molecular biology and developing or adopting new methods as needed.