Zooming into the fish's brain-What is really going on! Connectomics analysis of larval zebrafish.

项目3 项目3的主要目标是更好地将斑马鱼的行为与潜在的突触电路联系起来。在这两个 中枢神经系统和外周神经系统我们将利用高通量连续切片电子的最新进展 显微镜获取高分辨率的图像数据，然后使用新的机器学习方法来分析这些数据。这 努力将使用三种方法：1)稀疏地手动重建与特定路径相关的特定路径 行为，2)突触连接的密集重建，以揭示神经元的所有空间输入和输出 与显示兴奋性和抑制性细胞类型和活性的标记相关，以及3)饱和重建，也 提供神经胶质、血管、感觉末梢器官和肌肉(心脏、骨骼和肌肉)。 斑马鱼是唯一一种对整个神经系统进行饱和重建的脊椎动物 可想而知。连接数据最初将用于测试已发布的行为模型的有效性 在神经系统中实例化。在可能发生的情况下，基于 公布的模型和发现的，这些数据将被用来帮助制定新的模型。其次，将使用数据 揭示以前未知的通路，例如那些连接自主神经系统和躯体神经系统的通路。 最后，我们将构建必要的基础设施，以便将数据用作公共的、可自由寻址的地图集 对追踪构成脊椎动物行为基础的长距离和短距离神经通路感兴趣的研究人员 单个突触的分辨率。

Project 3 The principal goal of Project 3 is to better link the behaviors of zebrafish to underlying synaptic circuitry. In both the central and peripheral nervous systems we will leverage recent advances in high throughput serial section electron microscopy to acquire high resolution image data and then use new machine learning approaches to analyze this data. This effort will use three kinds of approaches: 1) sparse manual reconstructions of particular pathways related to particular behaviors, 2) dense reconstruction of synaptic connections to reveal all the input and output of neurons that is spatially correlated to markers that show excitatory and inhibitory cell types and activity, and 3) saturated reconstructions that also provide glial, vascular, sensory end organs and muscle (cardiac, skeletal and smooth). The zebrafish is the only vertebrate animal where obtaining a saturated reconstruction of the entire nervous system is conceivable. The connectomic data will be used initially to test the validity of published models of how a behavior is instantiated in the nervous system. In the likely event that there is some difference between what was expected based on published models and what is found, these data will be used to help formulate new models. Second, the data will be used to reveal previously unknown pathways, such as those that interconnect the autonomic and somatic nervous systems. Finally we will build the necessary infrastructure so the data can be used as a public, freely addressable atlas for any researcher who has interest in tracking the long and short range neural pathways that underlie vertebrate behavior at the resolution of single synapses.