Lightsheet Microscope

项目概要/摘要： 大样本三维成像技术正成为识别和分析生物样品的重要技术 复杂的细胞迁移模式，血管完整性，发育细胞模式和神经 电路的复杂性，所有的研究在刘易斯卡茨医学院的基本组成部分， Temple（LKSOM）.为了支持这些研究工作， 申请资金购买光片显微镜这种光片显微镜将是一种重要的 除了过去10年通过赠款资助支持的成像核心， Shriners儿科研究医院。成像核心的使命是提供一个完整的 一系列最先进的成像设备、培训和技术，具有成本效益。 成像核心目前具有2个共焦、1个多光子、TIRF、体视学系统、激光器 捕获，和3个宽场epiflucentscope，与20多名研究人员在Shriners研究 中心，LKSOM和坦普尔大学，他们在他们的出版物中承认了这一核心 以及超过45个联邦或私人基金赠款。 光片显微镜是成像核心的重要和必要的补充， 支持我们目前的成像设备所不能实现的高分辨率3D成像。它将有 对目前PHS资助的项目以及生物医学产生根本性和重大影响 通过高影响力的出版物传播发现。对光片的需求 显微镜是通过与多名研究人员的互动和合作开发的， 这项技术的需求，以显着提高他们目前和未来的研究工作。 此外，这种共享的工具也将提高我们招募新教师的能力， 他们的研究项目迫切需要3D成像。与所有高端成像一样， 仪器，个别实验室没有足够的资金购买或维护光片 显微镜因此，它是多个研究者共享的高度相关的工具 并得到一个拥有10年经验和资金的既定成像核心的支持， 有资格操作、管理和维护本显微镜。

Project Summary/Abstract: Three-dimensional imaging of large samples is becoming a vital technology for identifying complex cell migration patterns, vascular integrity, developmental cell patterning and neural circuit complexity, all essential components of research at Lewis Katz School of Medicine at Temple (LKSOM). To support these research efforts, this shared instrument application requests funds for a lightsheet microscope. This lightsheet microscope will be an important addition to the Imaging Core supported over the past 10 years by grant funding through Shriners Hospitals for Pediatric Research. The mission of the imaging core is to provide a full range of state-of-the-art imaging equipment, training, and technology at a cost-effective rate. The imaging core presently has a 2 confocal, 1 multi-photon, TIRF, stereological system, laser capture, and 3 wide-field epifluorescent scopes, with over 20 researchers at Shriners Research Center, LKSOM, and Temple University who have acknowledged this core in their publications and in over 45 federal or private foundation grants. The lightsheet microscope is an important and necessary addition to the imaging core by supporting high resolution 3D imaging not capable by our present imaging devices. It will have a fundamental and significant impact on currently PHS funded projects as well as biomedical discoveries disseminated through high impact publications. The need for a lightsheet microscope developed through interactions and collaboration with multiple investigators for the need of this technology to significantly enhance their current and future research efforts. Additionally, this shared instrument will also enhance our ability to recruit new faculty who have a critical need for 3D imaging for their research program. As with all high-end imaging instrumentation, individual labs have insufficient funds to purchase or maintain a lightsheet microscope. Therefore, it is highly relevant instrument to be shared by multiple investigators and supported by an established imaging core with 10 years of experience and funding, qualified to operate, manage and maintain this microscope.