Pacific Northwest Center for Cryo-EM: 2023 screening supplement

项目摘要（来自原始申请） 冷冻电子显微镜（cryo-EM）彻底改变了生物医学科学中的成像，从近原子 通过单粒子冷冻EM对单个生物分子的分辨率可视化， 大分子复合物，在原位，使用低温电子断层扫描（cryo-ET）。这场革命 这主要是由于用于收集冷冻EM图像的探测器的进步， 用于图像分析和样品重建的程序，以及显微镜本身。 因为支撑冷冻EM的技术既昂贵，又在短时间内取得了显着进步， 在一段时间内，许多研究小组可以从将cryo-EM纳入其生物医学研究中受益 既不能接触到冷冻EM技术，也没有接受过使用该技术的培训。解决访问问题 我们将建立西北太平洋冷冻EM中心。PNCC将 是俄勒冈州健康与科学大学（OHSU）和太平洋西北部之间的合作努力 国家实验室（PNNL）。通过利用OHSU在高分辨率成像方面的优势， 样品制备和教育在冷冻电镜与长期的经验，PNNL在电子 显微镜，高性能计算和强大的用户设施操作，我们将赋予PNCC 在单粒子冷冻EM和冷冻ET方面的卓越能力，以及全面和完整的 冷冻EM教育或交叉培训计划。此外，由于PNCC已经建立了合作关系， 由于在低温电磁技术和计算机硬件及软件方面处于领先地位，PNCC将走在最前沿 技术能力。此外，OHSU参加了由联合国共同赞助的冷冻EM学校， 显微镜制造商FEI将支持PNCC的交叉培训计划。OHSU和PNNL将 对PNCC进行重大投资。OHSU将为仪表提供低振动空间， 向中心捐赠一台新的显微镜，OHSU将提供1900万美元的额外资金用于太空 翻修、购置额外的显微镜和支助中心工作人员。与此同时，PNNL已 承诺进入他们的高性能计算机中心，他们的用户程序和高分辨率 电子显微镜通过将两个优势互补的主要研究机构聚集在一起， 通过明智选择的合作和主要的额外支持来源，我们将建立一个国家- 最先进的，以用户为中心的生物分子和大分子高分辨率成像中心 这将有助于我们的组装和下一代科学家在冷冻EM领域的培训教育。

PROJECT SUMMARY (from original application) Cryo-electron microscopy (cryo-EM) has revolutionized imaging in biomedical science, from near atomic resolution visualization of individual biological molecules by single particle cryo-EM to the definition of macromolecular complexes, in situ, using cryo-electron tomography (cryo-ET). This revolution has been drivenlargely by advances in the detectors employed to collect the cryo-EM images, in the computer software programs utilized in image analysis and specimen reconstruction, and in the microscopes themselves. Because the technology underpinning cryo-EM is both expensive and has dramatically advanced over a short period of time, many research groups that could benefit from incorporating cryo-EM in their biomedical studies neither have access to cryo-EM technology nor have the training to employ the technology. To address access to cryo-EM technology and education, we will create the Pacific Northwest Center for Cryo-EM. The PNCC will be a collaborative effort between the Oregon Health and Science University (OHSU) and the Pacific Northwest National Laboratory (PNNL). By harnessing the strengths of OHSU in high resolution imaging, biological sample preparation and education in cryo-EM with the long-standing experience of PNNL in electron microscopy, high performance computing and robust user facility operation, we will endow the PNCC with outstanding capabilities in single particle cryo-EM and cryo-ET, as well as with a thorough and complete program in cryo-EM education or cross-training. Moreover, because the PNCC has established collaborations with leaders in cryo-EM technology and in computer hardware and software, the PNCC will be at the forefront of technological capability. Furthermore, the participation of OHSU in a cryo-EM school co-sponsored by the microscope manufacturer, FEI, will bolster the cross-training program of the PNCC. Both OHSU and PNNL will make major investments in the PNCC. OHSU will provide a low vibration space for the instrumentation, it will donate a new microscope to the center and OHSU will provide $19M in additional funding for use in space renovation, acquisition of an additional microscope and support of center staff. At the same time, PNNL has pledged access to their high-performance computer centers, their user programs and their high-resolution electron microscopes. By bringing together two major research institutions with complementary strengths, together with judiciously chosen collaborations and major additional sources of support, we will create a state- of-the-art, user-focused center for the high-resolution imaging of biological molecules and macromolecular assemblies and for the education of the next generation of scientists trained in the field of cryo-EM.