OPTICAL BIOPSY USING OPTICAL COHERENCE TOMOGRAPHY

该计划是一个多学科的合作努力，这是一个 继续进行有调查人员参与的研究 麻省理工学院和麻省总医院 医院。这一提议的假设是光学相干性 层析成像--一种新的原位光学诊断成像技术 组织微结构成像，可以开发和应用于 “光学活组织检查”早期的原位诊断和监测 肿瘤性改变。拟议的计划将包括几个 目标相辅相成的组成部分。研究将在以下情况下进行 现有的OCT技术以15-20的图像分辨率运行 微米用于检查不同器官组织的肿瘤性变化 系统，并与组织病理学建立对应关系。尽管 一些肿瘤性变化，如腺体肥大，可能会 在这种分辨率下发生的一些恶性肿瘤，如子宫颈 癌症，可能需要更高的分辨率才能实现核电 分析。将开发OCT技术，以扩展 将成像分辨率限制在3-4微米级以实现这一点 细胞和亚细胞水平的成像。高分辨率成像 然后将进行研究并与组织病理学通信 在选定的组织病理学上进行研究，以便评估 可视化微结构变化对识别肿瘤至关重要 转型。我们希望以3-4微米的分辨率成像 应该是朝着广泛的诊断范围迈出的一步 申请。为了发展体内实验的基础 研究，我们将开发一种前向扫描刚性内窥镜和一种 前向扫描软性导管/内窥镜。连同一个 我们已经开发的横向扫描OCT导管，我们 将对胃肠道正常组织进行活体成像研究， 正常动物的尿路、生殖道和呼吸道 模特。这些研究将确定必须解决的问题 活体成像研究及其在体外和体外的可能差异 活体成像。最后，我们将进行活体成像研究 用仓鼠颊囊癌模型评价其治疗能力 OCT对体内癌前病变和癌变进行成像。已被占用 总而言之，这些研究有望开发一种新的成像技术 诊断，这可能是一个强大的工具在诊断和 预防恶性疾病。

The program is a multidisciplinary, collaborative effort which is a continuation of ongoing research involving investigators at the Massachusetts Institute of Technology and the Massachusetts General Hospital. The hypothesis of this proposal is that optical coherence tomography, a new optical diagnostic imaging technology for in situ imaging of tissue microstructure, can be developed and applied for "optical biopsy," the in situ diagnosis and monitoring of early neoplastic changes. The proposed program will consist of several components with complementary aims. Studies will be performed with existing OCT technology operating at image resolutions of 15-20 microns to examine neoplastic changes in tissue from various organ systems and establish correspondence with histopathology. Though some neoplastic changes such as glandular hypertropy are likely to occur at this resolution, some malignancies, such as cervical carcinoma, will likely require highter resolution to allow nuclear analysis. OCT technology will be developed in order to extend the limit of imaging resolution to the 3-4 micron scale to permit this cellular and subcellular level imaging. High resolution imaging studies and correspondence with histopathology will then be performed on selected tissue pathologies in order to evaluate the ability to visualize microstructure changes critical in identifying neoplastic transformation. We expect that imaging at 3-4 micron resolution should be an enabling step toward a wide range of diagnostic applications. In order to develop the foundation for in vivo studies, we will develop a forward scanning rigid endoscope and a forward scanning flexible catheter/endoscope. Together with a transverse scanning OCT catheter that we have already developed, we will perform in vivo imaging studies of normal tissue of the GI, urinary, reproductive, and respiratory tracts in a normal animal model. These studies will identify issues which must be addressed in vivo imaging studies and possible differences between in vitro and in vivo imaging. Finally we will perform in vivo imaging studies using the hamster cheek pouch carcinoma model to evaluate the ability of OCT to image premalignant and malignant changes in vivo. Taken together, these studies hold the promise of developing a new imaging diagnostic which could be a powerful tool in the diagnosis and prevention of malignancy.