LOW VISCOSITY SEPARATION MEDIA FOR DNA SEQUENCING

目前的高通量DNA测序系统可以产生序列 数据以每年大约29兆数据库的理论速度增长。 毛细管阵列电泳(CAB)系统可以提高DNA 按当前数量级对吞吐量进行排序 可用的系统。可流动的分离介质是关键之一 驾驶室系统的组成和分离介质的改进 大大降低了出租车系统的成本。中等至偏高 前人研究人员使用的分离介质的粘度 这使得设计加压毛细管成为一项艰巨的任务 商用出租车系统的加油站。第一阶段的目标是 这项建议的目的是开发低粘度介质，这将使 使单基分辨率达到1000基数。vbl.使用 低粘度分离介质将简化压力容器 驾驶室系统的设计问题，导致大量 降低成本。使用这种高吞吐量的驾驶室系统将 使大规模测序项目发生革命性变化，并大幅 降低每个基地的成本。第二阶段研究将扩大该设施的使用范围 低粘度和高分辨率的介质，价格较低 动态涂层毛细血管，无需 高成本的共价涂层毛细管。这将进一步减少 每个系统和每个碱基序列的成本。 建议的商业应用：CAE系统具有重要的 通过以下方式改善美国经济增长和生产率的潜力 加快新基因信息产生的速度。 可靠、经济高效的测序和基因分型将具有 对人类基因组计划、医疗保健、 农业综合企业、法医分析和制药行业。

Current high-throughput DNA sequencing systems can produce sequence data at a theoretical rate of approximately 29 megabases per year. Capillary Array Electrophoresis (CAB) systems can increase the DNA sequencing throughput by an order-of-magnitude over currently available systems. A flowable separation medium is one of the key components of the CAB system, and improvements in separation media significantly lower the cost of a CAB system. The moderate-to-high viscosities of the separation media used by previous researchers makes it a formidable task to design a pressurized capillary refilling station for a commercial CAB system. The goal of Phase I of this proposal is to develop low-viscosity media which will enable single base resolution out to 1000 bases. Using low-viscosity separation media will simplify the pressure-vessel design issues of the CAB system, resulting in a substantial reduction in cost. Use of such high-throughput CAB systems will revolutionize large-scale sequencing projects, and substantially reduce the cost per base. Phase II studies will extend the utility of low-viscosity and high-resolution media with less expensive dynamically-coated capillaries by eliminating the need for high-cost covalently-coated capillaries. This will further reduce the cost per system and per base sequences. PROPOSED COMMERCIAL APPLICATIONS: A CAE system has significant potential to improve the U.S. economic growth and productivity by accelerating the rate that new genetic information is generated. Reliable, cost-efficient sequencing and genetic typing will have significant impact on Human Genome Project, healthcare, agribusiness, forensic analysis, and pharmaceutical industry.