CORE-- MORPHOLOGY

本核心检讨将继续以促进和确保个别项目的具体目标得以实现为总体目标。此外，该中心还将在华盛顿大学提供集中设施，用于神经元染色、超微结构分析和用于多个项目的实验动物的分布。因此，核心将：1)为所有组成项目提供技术专长；2)最佳利用个体实验动物；3)确保从不同实验室获得的数据具有可比性，并以类似的方式进行分析，以避免独立观察者和不同分析技术所获得的结果相互关联所带来的常见陷阱；5)提供实验和概念整合和外部审查的手段。为了实现这些具体目标，该核心将执行5个不同的功能。它们是：1；位于圣路易斯的单一设备，用于在各种实验准备中染色体感神经元的远程投射和树突- CORE将在圣路易斯运营一个设备，该设备将用于项目#s1、2、4和5，用于染色体感神经轴不同水平的单个神经元的过程。2. 圣路易斯的单一电子显微镜设备用于组织处理和超微结构的记录-圣路易斯的两个项目（#s 1和4）需要电子显微镜。我们建议继续由Joseph DeMaro先生担任CORE EM技术人员和相关的原位设备，为神经营养因子增强和干细胞移植条件下的中枢超微结构发育研究提供途径。3. 在不同的实验室之间及时传递数据和想法——包含本提案的项目在技术上和概念上都是密切相关的。预定的双月会议将确保在实验室之间定期传递必要的信息、材料和重要评论。4. 持续检讨工作进展及结果-我们会成立新的及适当的内部及外部谘询小组，每年举行会议，评估我们的工作，以达致上述目标。神经发育领域的专家也将被邀请作报告，并与项目调查人员进行非正式的访问。5. 所有必要的和人员目前都在没有我们的计划项目的情况下运作。

This CORE will continue to have as its general aim to facilitate and ensure the accomplishment of the individual projects' Specific Aims. In addition, the Core will provide centralized facilities at Washington University for staining neurons, ultrastructural analyses, and distribution of experimental animals that are used in more than one project.. As such, the CORE will: 1) make technical expertise available to all of the component projects, 2) make optimum use of individual experimental animals, 3) ensure that the data obtained from the different laboratories are comparable and analyzed in a like manner so as to circumvent common pitfalls associated with the correlation of findings obtained by independent observers and different analytic techniques, 4) administer the various projects, and 5) provide means for experimental and conceptual integration and external review. To accomplish these Specific Aims, this CORE will carry out 5 different functions. These are: 1. A single facility in St. Louis for staining the long-range projections and dendrites of somatosensory neurons in various experimental preparations- The CORE will operate a facility in St. Louis that will be used by Project #s1, 2, 4, and 5 to stain the processes of single neurons at various levels of somatosensory neuraxis. 2. A single electron microscopy facility in St. Louis for tissue processing and documentation of ultrastructure- Two projects in St. Louis (#s 1 and 4) require electron microscopy. We proposed to continue with Mr. Joseph DeMaro as the CORE EM technician and associated in place equipment to provide an avenue for study of central ultrastructural development under conditions of neurotrophin augmentation and stem cell transplantation. 3. Timely transmission of data and ideas between the different laboratories-The projects comprising this proposal are closely interrelated technically and conceptually. Scheduled bimonthly meetings will ensure that necessary information, materials and critical reviews are transmitted between laboratories on a regular basis. 4. Continual external review of progress and results-This aim will continue to be met by establishing new and appropriate internal and external advisory groups that will meet annually to evaluate our work. Experts in the field of neuronal development will also be invited to give presentations and visit less formally with project investigators. 5. Administration All requisite and personnel are currently functioning without our Program Project.