The Norwegian Mother And Child Study: Environmental Spec

我的研究重点是通过改进暴露测量来评估常见环境暴露对健康的影响。在努力创造资源，以加强接触测量，我一直参与在挪威的一个大型出生队列的背景下创建一个生物标本库，以创建一个生物标本库，以评估环境暴露和健康影响孕妇及其未出生的孩子。我们预计在3-4年内招募50，000名女性及其后代。 我们目前正在进行试点工作，以评估防止尿液样本细菌污染的方法，同时仍然保持未来感兴趣的分析物（酚类，邻苯二甲酸酯，非持久性农药，砷，汞）。在与挪威研究人员的合作中，我们正在设计一个质量保证实验和协议，以评估各种参数的储存条件。这将需要大约2年的时间才能完成。我们还设计了一项研究，使用NIEHS样本并链接到母亲的问卷数据和医学出生登记数据，以评估常见环境污染物（可能是邻苯二甲酸酯）对健康的影响。 将在妊娠第17周采集女性的全血、血浆和尿液样本。目前正在执行严格的质量保证和质量控制计划，以确保样本质量，防止潜在的环境污染。每名女性的样本将包括1 × 2 ml EDTA全血真空采血管，用于在-20 ℃下储存的痕量金属分析，约3 ml肝素管血浆，储存在-80 ℃下，10 ml尿液，储存在-80 ℃下。这一资源将与挪威母亲和儿童群组研究中关于妇女和儿童的更多数据相联系。 已制定样本采集和储存方案，并经各方同意。参与NIEHS已获得IRB批准，样本采集已开始。

My research focuses on assessing health effects of common environmental exposures through improved exposure measurement. In an effort to create resources to enhance exposure measurement, I have been involved in the creation of a biological specimen repository in the context of a large birth cohort in Norway to create a biological specimen repository to evaluate environmental exposures and health effects to pregnant women and their unborn children. We anticipate enrolling 50,000 women and their offspring over 3-4 years. We are currently conducting pilot work to assess means to prevent bacterial contamination of urine samples while still maintaining the analytes of future interest (phenols, phthalates, non-persistent pesticides, arsenic, mercury). In collaboration with Norwegian investigators, we are designing a quality assurance experiment and protocol to assess storage conditions for a variety of parameters. This will take ~2 years to complete. We are also designing a study to use the NIEHS samples and link to the questionnaire data from the mother and the Medical Birth Registry data to assess health effects of a common environmental contaminant, probably phthalates. Whole blood, plasma and urine samples will be collected from women at the 17th week of gestation. A rigorous quality assurance and quality control plan is being implemented to ensure sample quality with regard to potential environmental contamination. Samples from each woman will include 1x2ml EDTA vacutainer of whole blood for trace metal analysis stored at -20oC, ~3 ml of plasma from a heparin tube stored at -80oC, and 10 ml of urine stored at -80oC. This resource will be linked to additional data on the women and children in the Norway Mother and Child cohort study. The sample collection and storage protocol has been developed and agreed upon by all parties. IRB approval has been received for NIEHS participation and sample collection has begun.