CELL CULTURE CORE FACILITY

这个子项目是许多利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 维持哺乳动物和其他真核细胞在培养物中的能力是细胞免疫和生理反应研究以及感染因子如病毒和结核分枝杆菌研究的重要组成部分。 需要专门的培养设备和培养箱来维持这种培养。 该提案提供了一个研究人员可访问的，管理的核心设施，为边境生物医学研究中心的研究人员提供设备和技术支持，其项目依赖于细胞培养。 设备基础设施将包括生物安全柜、二氧化碳培养箱、深冻和超低温储存设施、倒置和复合显微镜以及超纯水供应。 这种支持对于支持研究毒物对细胞的影响、细胞免疫反应以及HBV和分枝杆菌的研究是必不可少的。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The ability to maintain mammalian and other eukaryotic cells in culture is an essential part of studies on cellular immune and physiological responses as well as investigations into infectious agents such as viruses and Mycobacterium tuberculosis. Specilized culturing equipment and incubators are required to maintain such cultures. This proposal provides for an investigator accessible, managed core facility to provide equipment and technical support for Border Biomedical Recearch Center researchers whose projects rely on cell culture. Equipment infrastructure will include hiosafety cabinets, CO2 incubators, deep freeze and ultra low temperature storage facilities, inverted and compound microscopes, and ultrapure water supply. Such support is and will be essential for the support of research into the effects of toxicants on cells, cellular immune responses, and studies on HBV and mycobacteria.