INFRASTRUCTURE 1: CORE RESEARCH LABORATORIES

这个子项目是许多利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 在塔斯基吉大学的CBR/RCMI核心设施的目的是促进多学科研究的研究人员使用显微镜/成像，流式细胞术，细胞培养和分子生物学，生物信息学和计算生物学。核心设施已向代表3所学院和9个科学学科的用户提供了服务和帮助。这些研究者发表或提交了4篇同行评审论文，并提交了5篇论文或海报。 成像设施被最多的研究者使用，而细胞培养和分子生物学设施继续积累最多的“工时”。 荧光显微镜，加上一个Q成像CE数码相机，通过C成像系统简单PCI软件操作，已被几个研究者使用。 此外，该相机应允许记录化学发光凝胶，而无需使用射线照相胶片。 通过购买几种最先进的仪器，分子生物学能力得到了显著提高。 其中包括BIO-TEK SYNERGY HT酶标仪，可读取6至384孔板，并进行荧光、吸光度和发光测量。 添加了Eppendorf主循环仪，更新了传统PCR。 购买了Cepheid Smartcycler 2，可提供出色的实时PCR功能。 该仪器采用4个光学通道;发射范围为510 nm至750 nm。 这些通道允许同时检测四个“探针”。 该仪器可实时显示生长曲线、温度曲线和熔解曲线，并进行定性、定量和生长曲线分析。 生物信息学和计算生物学设施致力于通过使用基于网络的基础设施，特别是在基因组学和蛋白质组学方面，增加教师在生物信息学和计算生物学研究中的参与。 我们将继续更新软件从martyrys。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The purpose of the CBR/RCMI Core Facility at Tuskegee University is to facilitate multidisciplinary research for investigators using Microscopy/Imaging, Flow Cytometry, Cell Culture and Molecular Biology, and Bioinformatics and Computational Biology. the Core Facility has provided service and assistance to users representing 3 academic colleges and 9 scientific disciplines. These investigators published or submitted 4 peer reviewed papers and presented 5 papers or posters. The Imaging Facility is used by the largest number of investigators while the Cell Culture and Molecular Biology facilities continue to accumulate the most "man hours" of use. Fluorescence microscopy, with the addition of a Q Imaging CE digital camera, operating through C Imaging Systems Simple PCI software, has been used by several investigators. Additionally, this camera should allow the recording of chemoluminescent gels without the use of radiographic film. Molecular biology capabilities have been significantly enhanced through the purchase of several state of the art instruments. These include a BIO-TEK SYNERGY HT microplate reader, which reads 6-to-384 well plates and performs fluorescence, absorbance and luminescence measurements. An Eppendorf Mastercycler has been added updating conventional PCR. A Cepheid Smartcycler 2 has been purchased and provides outstanding Real-time PCR capabilities. The instrument employs 4 optical channels; emissions range from 510 nm to 750 nm. These channels permit simultaneous detection of four "probes". The instrument provides real-time display of growth curves, temperature profiles, and melting curves with qualitative, quantitative, and growth curve analysis. The Bioinformatics and Computational Biology Facility strives to increase participation of faculty in bioinformatics and computational biology research through the use of web based infrastructure, especially in genomics and proteomics. We continue to update software from Accelrys.