Plant Nutrient-Growth Signaling Network

营养信号整合和协调基因表达、新陈代谢和生长。在……里面 多细胞生物、生长因子和激素在没有促进生长的情况下无效 营养信号网络的支持。然而，令人惊讶的是，人们对初选的了解很少 植物和动物中的营养信号机制。植物在连接人类与地球之间的 全球氮循环中无机氮向有机氮的同化转化 产生氨基酸、核酸和有机氮碳分子的无机硝酸盐， 对于建立和维持从植物到人类的生命是必不可少的。尽管基本的和 硝酸盐在基因表达、代谢、生长和发育中的多方面调节作用， 硝酸盐信号的分子和细胞机制在多细胞中仍然很难捉摸。 植物。由于基因冗余和突变致命性的阻碍，经典的遗传筛选受到了限制 在过去的二十年里，成功地识别了植物中关键的硝酸盐信号成分。通过 将分子、细胞、生化、功能基因组、化学遗传和 系统分析，我们发现了一种令人惊讶的分子联系，在特定的钙传感器 蛋白激酶(CPK)与结瘤起始蛋白(NLP)转录因子 作为植物硝酸盐信号网络的主要调节者。我们的研究表明 硝酸盐作为中心信号分子在转录组重新编程中的独特作用 地上部-根部协调，以塑造器官生物量和结构。我们最近还发现了 首个具有转录激活剂双重功能的植物硝酸盐传感器NLP7，以及组合 多个NLP在控制初级硝酸盐反应(PNR)中的作用 植物的根和地上部发育。我们计划在我们的新发现和创新的基础上 阐明营养生长网络的分子和细胞基础的实验平台 它协调整个系统的转录并调节植物的发育过程。我们会 整合互补的策略和方法，以促进我们对营养的理解 针对三个特定目标的信号机制： 目的：1.阐明NLP7硝酸盐传感器复合体的功能和作用 目的2.剖析硝酸盐引发的细胞内钙信号转导机制 目标3.发现硝酸盐信令中的CPK-TOR链路 为解开硝酸盐信号机制而提出的研究将在 营养感受器复合体的作用、营养介导的钙信号以及转录 以及具有超越植物生物学的持续科学影响的发育调控。

Nutrient signaling integrates and coordinates gene expression, metabolism, and growth. In multicellular organisms, growth factors and hormones are ineffective in growth promotion without the support of nutrient signaling networks. However, surprisingly little is known about the primary nutrient signaling mechanisms in plants and animals. Plants play a central role in bridging the conversion of inorganic nitrogen to organic nitrogen in the global nitrogen cycle by assimilating inorganic nitrate to generate amino acids, nucleic acids, and organic nitrogen-carbon molecules, which are essential to build and sustain lives from plants to humans. Despite the fundamental and multifaceted regulatory roles of nitrate in gene expression, metabolism, growth, and development, the molecular and cellular mechanisms of nitrate signaling remain largely elusive in multicellular plants. Hampered by gene redundancy and mutant lethality, classical genetic screens had limited success in identifying key nitrate signaling components in plants over the past two decades. By taking integrated molecular, cellular, biochemical, functional genomic, chemical genetic, and systems analyses, we have discovered a surprising molecular link between specific Ca2+-sensor protein kinases (CPKs) and the NODULE INCEPTION-LIKE PROTEIN (NLP) transcription factors as the primary regulators of the nitrate-signaling network in plants. Our research has demonstrated the unique role of nitrate as a central signaling molecule in transcriptome reprogramming and shoot-root coordination to shape organ biomass and architecture. We also recently discovered the first plant nitrate sensor NLP7 with a dual function as a transcription activator, and the combinatorial actions of multiple NLPs in controlling the primary nitrate responses (PNR) central to coordinate plant root and shoot development. We propose to build on our new findings and innovative experimental platforms to elucidate the molecular and cellular basis of the nutrient-growth network that orchestrates system-wide transcription and modulates plant developmental processes. We will integrate complementary strategies and methodologies to advance our understanding of nutrient signaling mechanisms for three specific aims: Aim1. Elucidate the function and action of the NLP7 nitrate sensor complex Aim 2. Dissect the intracellular Ca2+ signaling mechanism triggered by nitrate Aim 3. Uncover the CPK-TOR link in nitrate signaling The proposed research to unravel the nitrate signaling mechanisms will establish new paradigms in the action of nutrient sensor complexes, nutrient-mediated Ca2+ signaling, as well as transcriptional and developmental regulation with sustained scientific impact beyond plant biology.