Spatial genomics analysis of tumor samples

摘要 肿瘤微环境成像(TME)是了解肿瘤生长、疾病的关键 进展和治疗方案。TME通常由一个复杂的、不同的 免疫细胞、成纤维细胞和肿瘤细胞群。人们对“地理学”知之甚少 肿瘤和不同细胞类型的空间组织。快速识别的能力 使用分子标记的不同细胞类型和肿瘤亚型将对癌症产生转化作用 诊断。 在空间基因组公司，我们正在商业化允许在肿瘤中进行转录图谱分析的技术 样本。利用这项技术，可以直接识别原位的像元类型和空间组织 并在肿瘤组织切片中可见。这些功能提供了一个强大的工具来检查 肿瘤微环境中不同细胞类型及其相互作用。在这项提案中，我们将 开发试剂、仪器和相关软件，使研究人员能够 在肿瘤切片上进行空间映射实验。

SUMMARY Imaging the tumor microenvironment (TME) is key to understanding tumor growth, disease progression and therapy options. The TME often consists of a complex neighborhood of diverse populations of immune cells, fibroblasts, and tumors cells. Little is known about the “geography” of the tumors and the spatial organization of the distinct cell types. The ability to rapidly identify different cell types and tumor subtypes using molecular markers will be transformative for cancer diagnostics. At Spatial Genomics, we are commercializing technology that allows transcript profiling in tumor samples. With this technology, cell types in situ and spatial organizations can be directly identified and visualized in tumor tissue slices. These capabilities provide a powerful tool for examining the different cell types and their interactions in the tumor microenvironment. In this proposal, we will develop the reagents, instrumentation, and the associated software that will allow researchers to perform spatial mapping experiments in tumor slices.