Developmental Project Competition

B.3.b.发展项目。第一年选定了五个发展伙伴供资 中西部RCE行动假设发展中国家每年的直接费用预算为50万美元， Midwestem RCE将发布另一个RFA，以选择1 - 2个额外的提案在第1年获得资助。那些 在第一年申请最高供资额（10万美元直接费用）的发展伙伴不能 在第二年的融资中继续。预计DP研究将为 编制新的供资提案，提交给中西部地区RCE（RP），NIAID，DOE， 国防部或国土安全部。DPI "中西部RCE科学家多米尼克·米西阿卡斯将搜寻B。炭疽 突变体的分泌缺陷的PA，LF，或EF和分析的毒力特性， 在动物疾病模型中的变异。DP2 "中西部RCE科学家格雷格波兰将分离 来自I类HLA-A2分子结合沟的牛痘病毒衍生的加工肽， 牛痘病毒感染的树突状细胞，并研究这些树突状细胞的物理和生化属性， 肽表位。DP3：Midwestem RCE科学家James Baker Jr.（一）证明能力， 痘病毒的纳米乳剂灭活动力学，（ii）确定纳米乳剂是否灭活 牛痘病毒是免疫原性的，并且（iii）确定纳米乳剂是否可以预防或减弱 小鼠实验性牛痘病毒感染。DP4：中西部RCE科学家Paula Traktman将 利用RNAi技术和表位标记技术， 复制和萌芽。DP5 "中西部RCE科学家托马斯扎赫特和达拉弗兰克将 构建F.土拉热菌菌株LVS和寻找突变体， 在体外巨噬细胞中存活。这些突变体，然后将分析在小鼠模型的气溶胶 感染的发病机制缺陷。在每年的秋季和春季，AC将发布请求 对于用新提案取代DP计划的申请，新提案可以要求100，000美元或更少 最多两年的资金（不超过10万美元）。

B.3.b. Developmental Projects. Five DPs have been selected for funding during the first year of Midwestem RCE operation. Assuming a budget of $500,000 direct costs per year for DPs, the Midwestem RCE will post another RFA to select 1-2 additional proposals for funding in year 1. Those DPs that requested the maximum amount of funding in year 1 ($100,000 direct costs) cannot be continued in the second year of funding. It is expected that DP research generates data for the preparation of new funding proposals, submitted either to the Midwestem RCE (RPs), to NIAID, DOE, DOD or DOHS. DPI" Midwestem RCE scientist Dominique Missiakas will search for B. anthracis mutants that are defective for the secretion of PA, LF, or EF and analyze the virulence properties of the variants in an animal model of disease. DP2" Midwestem RCE scientist Greg Poland will isolate Vaccinia virus-derived, processed peptides from the binding grooves of class I, HLA-A2 molecules of Vaccinia virus-infected dendritic cells and study the physical and biochemical attributes of these peptide epitopes. DP3: Midwestem RCE scientist James Baker Jr. will (i) document the ability and kinetics of nanoemulsion inactivation of poxviruses, (ii) determine whether nanoemulsion inactivated Vaccinia virus is immunogenic and (iii) determine whether nanoemulsion can prevent or attenuate experimental Vaccinia virus infection in mice. DP4: Midwestem RCE scientist Paula Traktman will use RNAi technology and epitagging to identify those poxvirus genes that are essential for virus replication and budding. DP5" Midwestem RCE scientists Thomas Zahrt and Dara Frank will construct a transposon library in F. tularensis strain LVS and search for mutants that are unable to survive in macrophages in vitro. These mutants will then be analyzed in a murine model of aerosol infection for defects in pathogenesis. In the fall and spring of each year, the AC will post Requests For Applications to replace DP programs with new proposals that can request either $100,000 or less and up to two years of funding (not to exceed $100,000).