RHINITIS IS CHRONIC FATIGUE SYNDROME (CFS)

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 慢性疲劳综合征(CFS)、波斯湾战争病(PGI)和纤维肌痛是相互重叠的症状复合体，没有客观的标志物或已知的病理生理机制。神经功能障碍很常见。巴拉纽克博士对脑脊液进行了评估，以找出与对照受试者相比，在这种CFS疾病谱中差异表达的蛋白质。将10例CFS、10例PGI和10例对照组(50 uL/例)的脑脊液标本合并为一组(队列1)。队列2的12名对照组和9名CFS受试者分别接受了体液(200mul/受试者)的评估。胰酶消化后，多肽被 通过毛细管色谱、四极杆飞行时间质谱仪、多肽测序、生物信息学蛋白质鉴定和统计分析进行分析。合并的CFS和PGI样本共享20个在合并的对照样本中未检测到的蛋白质(队列1 CFS相关蛋白质组)。多元Logistic回归 队列2的分析(GLM)检测到10个CFS个体和队列1的CFS相关蛋白质组共有的蛋白质，但在对照样本中未检测到。在一组精选的5种CFS相关蛋白中检测&gt；or=1可预测CFS状态，符合率为80%(Logistic模型)。这些蛋白质是α-1- 巨球蛋白、淀粉样前体蛋白1、角蛋白16、类粘蛋白2和色素上皮衍生因子。总体而言，115种蛋白质中有62种是新描述的。因此，这项研究在脑脊液中检测到一组相同的中枢神经系统、先天性免疫和淀粉样蛋白 有重叠的CFS、PGI和纤维肌痛的独立队列受试者。尽管证候名称和定义不同，但这项研究表明，这些慢性疾病的蛋白质组可能是相同的，因此可能是潜在的病理机制(S)。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Chronic Fatigue Syndrome (CFS), Persian Gulf War Illness (PGI), and fibromyalgia are overlapping symptom complexes without objective markers or known pathophysiology. Neurological dysfunction is common. Dr. Baraniuk assessed cerebrospinal fluid to find proteins that were differentially expressed in this CFS-spectrum of illnesses compared to control subjects. Cerebrospinal fluid specimens from 10 CFS, 10 PGI, and 10 control subjects (50 ul/subject) were pooled into one sample per group (cohort 1). Cohort 2 of 12 control and 9 CFS subjects had their fluids (200 mul/subject) assessed individually. After trypsin digestion, peptides were analyzed by capillary chromatography, quadrupole-time-of-flight mass spectrometry, peptide sequencing, bioinformatic protein identification, and statistical analysis. Pooled CFS and PGI samples shared 20 proteins that were not detectable in the pooled control sample (cohort 1 CFS-related proteome). Multilogistic regression analysis (GLM) of cohort 2 detected 10 proteins that were shared by CFS individuals and the cohort 1 CFS related proteome, but were not detected in control samples. Detection of >or=1 of a select set of 5 CFS related proteins predicted CFS status with 80% concordance (logistic model). The proteins were alpha-1- macroglobulin, amyloid precursor-like protein 1, keratin 16, orosomucoid 2 and pigment epithelium-derived factor. Overall, 62 of 115 proteins were newly described. This study therefore detected an identical set of central nervous system, innate immune and amyloidogenic proteins in cerebrospinal fluids from two independent cohorts of subjects with overlapping CFS, PGI and fibromyalgia. Although syndrome names and definitions were different, this study demonstrates that the proteome, and therefore potentially the presumed pathological mechanism(s), may be shared across these chronic disorders.