KANSAS U COBRE MOLECULAR BIOLOGY CORE

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目及 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 分子生物学核心整合并增强了我们现有的生物技术支持设施和微阵列设施的能力，这些设施在增强和扩展KUMC研究人员的研究计划方面具有成功的历史。该核心将通过提供具有成本效益的 寡核苷酸合成、DMA序列分析和微阵列芯片处理服务。在过去的两年里，我们中心80%的成员（16/20）和5名COBRE项目新的初级调查员中的4名使用了这些设施。五个中心的教师，没有利用这些 两个刚到校园，另一个将在2007年1月就职。仅在2006财政年度，这组研究人员的使用就占所有KUMC寡核苷酸合成的22%，DMA测序的97%和微阵列芯片分析的33%。这个设施将为所有COBRE的科学家 提供合成寡核苷酸、获得DMA序列测定和分析以及向中心研究人员提供微阵列芯片和芯片处理技术的服务。COBRE基金将资助初级研究人员和使用核心的中心成员的活动，该机构将继续为该设施提供重要的国家支持。生物技术支持设施由一名实验室经理和三名工作人员组成。其中一名人员还担任微阵列设施的项目经理。所有人都是所需方法的专家，并将与COBRE分子生物学核心顾问进行交流。最后，核心将通过提供BAG扩展到提供新技术 重组工程专业知识和援助。将聘请一名在这方面有经验的研究助理来实施这项服务。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Molecular Biology Core integrates with and enhances the capabilities of our existing Biotechnology Support Facility and Microarray Facility with histories of success in augmenting and extending the research programs of investigators at KUMC. This core will assist COBRE investigators by providing cost-effective services for the synthesis of oligonucleotides, DMA sequence analysis and microarray chip processing. Eighty percent of our center members (16/20) and 4 of the 5 COBRE project new junior investigators have utilized th ese facilities during the last two years. Of the five center faculty that have not utilized these facilities, two just arrived on campus and another will assume his position in January 2007. Usage by this group of researchers accounts for 22% of all KUMC oligonucleotide synthesis, 97% of DMA sequencing and 33% of microarray chip analysis during fiscal year 2006 alone. This facility will provide all COBRE scientists with services to synthesize oligonucleotides, obtain DMA sequence determination and analysis, and supply microarray chips and chip processing technologies to center investigators. The COBRE funds will subsidize activities for the junior investigators and for members of the center who use the core, and the institution will continue to provide significant state support for this facility. The Biotechnology Support Facility is staffed by a lab manager and three personnel. One of these personnel also serves as the project manager of the Microarray Facility. All are experts with the methods required and will interface with the COBRE Molecular Biology Core Advisor. Finally, the core will expand to provide new technology by offering BAG recombineering expertise and assistance. A Research Associate with experience in this area will be hired to implement and staff this service.