LOUISIANA COBRE: OBESITY & DIABETES RES: GENOMICS CORE

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者的研究机构。 彭宁顿生物医学研究中心基因组核心设施（PBRC-GCF）的目标是为路易斯安那州的LSI社区和周围机构提供高通量DNA测序、真实的时间PCR和微阵列能力。PBRC全球合作框架网站（http：//gcf.pbrc.edi/）。公开 在2001年春季，该区域的一些机构广泛使用该系统自动提交样品、检索数据和开帐单。该网站正在不断修改1：0适合研究人员在彭宁顿和周围的科学界的需求。我们配备了一台AB 1 3700（96毛细管）和两台ABI 3100（16毛细管）遗传分析仪，每天有能力测序超过50万个碱基对。PBRC-GCF微阵列设施从小鼠（Operon，13，442和最近更新的16，463寡核苷酸文库）和人（Compugen. 18，861寡核苷酸文库）和PBRC科学家产生的几个cDNA文库。用配备有服务器臂的GeneMachine OmniGrid微阵列仪打印载玻片，并使用GS1 Lumonics ScanArray 5000扫描。生物信息学支持也将提供给研究人员，我们是 目前正在开发微阵列数据和信息的数据库，该数据库遵循MIAMK概述的指导方针，MIAMK是一项国际努力，旨在标准化微阵列数据，以提供关于微阵列实验的最小信息。PBRC-GCF配备了机器人（Biomek FX机器人工作站，Beckman-Coulter），以支持高通量DNA测序和微阵列技术， 实时PCR序列检测系统（ABI 7700; ABI 7900）用于验证微阵列结果和定量RT-PCR。PBRC-GCF由Leslie P. Kozak博士和Robert A. KoZa由Susan纽曼在Rebecca McCabe的协助下管理。除了提供服务外，该中心还将开发新的方法，以加强建设和平研究中心科学家的研究活动。最初的发展将是一个CpG岛微阵列的利益，研究人员的cpigenetic变化与成人疾病。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The goal of the Pennington Biomedical Research Centers Genomlcs Core Facility (PBRC-GCF) ).s to provide high-throughput DNA sequencing, real time PCR and microarray capabilities for the LSI) community and surrounding institutions in the State of Louisiana. The PBRC-GCF website (http://gcf.pbrc.edii/). made public during the spring of 2001, has been used extensively for automated sample submission, data retrieval and billing by several institutions in the region. The website is continually being modified 1:0 suit the needs of research staff at Pennington and the surrounding scientific community. We are equipped with one AB1 3700 (96-capillary) and two ABI 3100s (16-capillary) genetic analyzer(s) and have the capability to sequence more than Vz million basepairs a day. The PBRC-GCF micro array facility produces high-density micro arrays from mouse (Operon, 13,442 and recently updated 16,463 oligonucleotide libraries) and human (Compugen. 18,861 oligonucleotide library) and several cDNA libraries generated by scientists at PBRC. Slides are printed with a GeneMachine OmniGrid Microarrayer equipped with server arm and scanned using a GS1 Lumonics ScanArray 5000. Bioinformatics support will also be available to investigators and we are presently developing databases for microarray data and information following the guidelines outlined by MIAMK, an international effort to standardize microarray data to provide minimum information about a microarray experiment. The PBRC-GCF is equipped with robotics (Biomek FX robotic workstation, Beckman-Coulter) to support the high-throughput DNA sequencing and microarray technologies, and, two real-time PCR sequence detection systems (ABI 7700; ABI 7900) for verification of microarray results and quantitative RT-PCR. The PBRC-GCF is directed by Dr. Leslie P. Kozak and Dr. Robert A. KoZa arid managed by Susan Newman with assistance from Rebecca McCabe. In addition to services, the Core will develop new methodologies that will enhance the research activities of PBRC scientists. The initial development will be a CpG island microarray for the benefit of researchers working on cpigenetic changes in relation to adult disease.