HIGH RESOLUTION STRUCTURAL STUDIES ON MACROMOLECULAR CRYSTALS

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 将为七个不同的晶体学项目收集同步加速器X射线衍射数据。 (1)一个突变的葡萄糖异构酶系统，无底物，底物结合和活性位点将被调查，以获得超高分辨率的结构。 这一努力将与计划于2001年11月在劳厄朗之万研究所进行的所有三个样品的中子衍射研究相协调，我们将在其中阐明酶的机制。(2)中等分辨率的同步加速器数据（>3 <$）将收集在bFGF/DNA复合物上，由于大的单位细胞边缘，该复合物迄今为止尚未进行实验室研究。 (3)将进行MAD研究以获得C1，肌联蛋白2-结构域片段的结构。（4）将使用马血清白蛋白研究微重力的影响并提高当前结构的分辨率。(5)最后，将对Der f2（尘螨过敏原）进行结构研究（6）RNA十二聚体，（7）RNA假结。 每个系统都具有工业、生物医学和马血清白蛋白研究的重要性。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Synchrotron X-ray diffraction data will be collected for seven different crystallographic projects. (1) A mutant glucose isomerase system, substrate free, substrate bound and active site will be investigated to obtain ultra-high resolution structures. This effort will be coordinated with a neutron diffraction study on all three samples scheduled for beamtime at the Institute Laue Langevin in November 2001 in which we will elucidate the enzymatic mechanism. (2) Medium resolution synchrotron data (>3 ¿) will be collected on a bFGF/DNA complex that has thus far eluded laboratory studies due to the large unit cell edge. (3) A MAD study will be carried out to obtain the structure of the C1, titin 2-domain fragment, (4) Equine serum albumin will be used for studying the effects of microgravity and to improve the resolution of the current structure. (5) Finally structural studies will be carried out on Der f2, a dust mite allergen (6) on an RNA dodecamer, and (7) on RNA pseudoknot. Each system has industrial, biomedical and in the case of the equine serum albumin study, fundamental importance.