MEASUREMENT OF ANALYTES IN WHOLE BLOOD AND SERUM

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 快速可靠地测量临床相关分析物的水平对于医生在对患者进行评估时是非常宝贵的的治疗选择。 目前的临床实验室检测涉及样品制备、试剂使用，并且是时间密集型的。 拉曼光谱法具有很高的特异性，可以同时测量多种分析物。 在过去的研究中，近红外拉曼已被证明能够检测低毫摩尔范围内的多种血液分析物。 然而，校准算法的前瞻性应用，其中分析物浓度预测独立的样品，并没有成功。 该可行性研究旨在重新评估NIR拉曼光谱检测限并证明成功的校准转移。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Rapid and reliable measurements of the levels of clinically-relevant analytes are invaluable to physicians when making assessments about a patients treatment options. Current clinical laboratory tests involve sample preparation, the use of reagents, and are time-intensive. Raman spectroscopy is attractive in that it can measure multiple analytes at once with high specificty. In past research, NIR Raman has proven capable of detecting multiple blood analytes in the low millimolar range. However, prospective application of the calibration algorithm, in which analyte concentrations are predicted for independent samples, has not been successful. This feasibility study seeks to both re-evaluate NIR Raman spectroscopy detection limits and to demonstrate successful calibration transfer.