REGULATION OF THE ACTIN CYTOSKELETON AND ENDOCYTOSIS IN BUDDING YEAST BY ARK1 A

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 肌动蛋白细胞骨架在细胞内吞、细胞运动、细胞形态和细胞器运输等重要的细胞过程中发挥作用。 肌动蛋白细胞骨架是一个动态的网络的细丝和相关的蛋白质，经历拆卸和重组，以响应各种细胞和细胞外信号。 丝氨酸/苏氨酸激酶Ark 1和Prk 1是最近发现的在酿酒酵母中将肌动蛋白细胞骨架的动态过程与内吞作用偶联的激酶。 这些激酶的哺乳动物同源物AAK 1和GAK也涉及哺乳动物细胞内吞作用的控制。 为了阐明Ark 1/Prk 1在肌动蛋白细胞骨架组装和内吞作用中的调控机制，我们正在利用化学遗传学/蛋白质组学策略来识别Ark 1和Prk 1的激酶靶点。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The actin cytoskeleton functions in a variety of important cellular processes including endocytosis, cell motility, cell morphology and organelle transport. The actin cytoskeleton is a dynamic network of filaments and associated proteins that undergoes disassembly and reassembly in response to various cellular and extracellular signals. The Ser/ Thr kinases, Ark1 and Prk1, were recently found to couple the dynamic processes of the actin cytoskeleton to endocytosis in Saccharomyces cerevisiae. The mammalian homologs of these kinases, AAK1 and GAK, are also implicated in the control of endocytosis in mammalian cells. To elucidate the regulatory mechanisms of Ark1/ Prk1 in the actin cytoskeleton assembly and endocytosis, we are exploiting chemical-genetic/ proteomic strategies to identify kinase targets of Ark1 and Prk1.