STRUCTURE DETERMINATION OF SEC13 AND NUP96 COMPLEX

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 核孔复合物（NPC）是由至少30个不同的蛋白质亚基组成的大分子结构。 NPC最明显的作用是作为大分子核质交换的唯一通道，无论是通过被动扩散还是促进运输。 NPC由三种不同的建筑结构组成：中央框架，细胞质丝和核篮。 中央框架被认为是形成连接细胞质和内核的通道。 NPC包含八重对称。 由于这种对称性，大多数核孔蛋白应该以8或8的倍数的拷贝数存在。 大多数核孔蛋白对称地位于NPC的两侧，而只有少数位于细胞核或细胞质表面。 对于NPC架构的完整图片，将需要高分辨率的三维结构。然而，一次获得关于完整结构的这样的图是不可行的。 Blobel实验室正在采取一种协作方法来构建这样一个模型。 我已经cocystalized核孔复合物的两个组件，Sec 13和Nup 96的片段。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The nuclear pore complex (NPC) is a large macromolecular structure composed of at least 30 different protein subunits. The most obvious roles of the NPC is being the sole gateway for nucleocytoplasmic exchange of macromolecules, either through passive diffusion or facilitated transported. The NPC consists of three distinct architectural structures: the central framework, the cytoplasmic filaments and the nuclear basket. The central framework is believed to form the channel that connects the cytoplasm and inner nucleus. The NPC contains an eight fold symmetry. Due do this symmetry, most nucleoporin should be present at a copy number of eight or a multiple of eight. Most of the nucleoporins are located symmetrically on both sides of the NPC, whereas only a few are positioned at either the nuclear or the cytoplasmic face. For a complete picture of the NPC architecture a high resolution three dimensional structure would be required. However, it is not feasible to obtain such a map on the complete structure at once. A collaborative approach taken by the Blobel lab to build such a model is underway. I have cocystalized two components of the nuclearpore complex, Sec13 and a fragment of Nup96.