Male accessory gland proteins of the dengue vector mosquito Aedes aegypti: potent

描述（由申请人提供）：在本研究中，我们将使用基因组学工具与田间研究的新组合，以解决登革病媒蚊子埃及伊蚊的雄性生殖蛋白（Acps为附属腺蛋白）的功能及其作为遗传控制策略靶点的潜力。雄性Acp首先在黑腹果蝇中被发现，在那里它们已经被证明对雌性的生殖生物学具有显着的影响。从雄性果蝇中鉴定出的Acps影响雌性果蝇的产卵和产卵、精子储存、交配和摄食行为。早期的研究与Ae。埃及伊蚊证明了“matrone”（一种部分纯化的雄性附属腺物质）对雌性行为和生理的影响。注入雌性Ae的Matrone或附属腺液。埃及伊蚊影响雌性的性不应性、吸血行为、血液消化、飞行、卵巢发育和产卵。这项早期的工作和我们自己的初步数据表明，男性Acps将是重要的Ae。因此，有必要详细调查这些设施，并确定其功能。我们将测试的假设，Ae。埃及伊蚊雄性在交配过程中将Acps转移给雌性，这些蛋白质对雌性蚊子的生物学和生殖适应性有重大影响。最终，这些Acps可能会影响各种女性特征，从而驱动病媒传播的疾病动态。公共卫生相关性在这项研究中，我们将确定登革热病媒蚊子，埃及伊蚊的雄性生殖蛋白。我们将研究这些蛋白质对雌性媒介生物学的影响，以及它们作为遗传控制策略靶点的潜力，通过实验室和田间研究的新组合。

DESCRIPTION (provided by applicant): In this study we will use a novel combination of genomics tools with field studies to address the function of male reproductive proteins (Acps for Accessory gland proteins) of the dengue vector mosquito, Aedes aegypti, and their potential as targets for genetic control strategies. Male Acps were first identified in Drosophila melanogaster, where they have been shown to have dramatic effects on the reproductive biology of the female. Individual Acps identified from male Drosophila affect female egg production and egg laying, sperm storage, mating and feeding behaviors. Earlier studies with Ae. aegypti demonstrated an effect of "matrone", a partially purified male accessory gland substance, on female behavior and physiology. Matrone or accessory gland fluid injected into female Ae. aegypti has affected female sexual refractoriness, blood feeding behavior, blood digestion, flight, ovarian development, and oviposition. This early work and our own preliminary data indicate that male Acps will be important in Ae. aegypti and makes it imperative to investigate them in detail and determine their function. We will test the hypothesis that Ae. aegypti males transfer Acps to females during mating, and that these proteins have a significant impact on the female mosquito biology and reproductive fitness. Ultimately, these Acps may influence a variety of female traits that drive vector-borne disease dynamics. PUBLIC HEALTH RELEVANCE In this study we will identify male reproductive proteins of the dengue vector mosquito, Aedes aegypti. We will investigate the impact of these proteins on female vector biology and their potential as targets for genetic control strategies though a novel combination of laboratory and field studies.