DEVELOPMENT OF RHESUS MONKEY MICROARRAYS

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 基因表达的微阵列分析技术尚未被最佳地应用于非人类灵长类动物的研究。一种广泛使用的微阵列分析工具是Affymetrix基因芯片，每个芯片可以分析超过15,000个mRNA。然而，由于Affymetrix芯片以短寡核苷酸为基础，单碱基错配将阻止杂交，因此人们普遍认为Affymetrix芯片不适合跨物种杂交，即使是像猴子和人类这样亲近的物种也不适合。在这个由ONPRC和内布拉斯加州大学合作的项目中，通过使用定向PCR技术生成恒河猴cdna序列，将促进特定恒河猴微阵列芯片的构建。到目前为止，已经产生了3000多个序列并存储到GenBank中。下一步将是将这些序列与其他可用的恒河猴DNA序列结合起来，设计出可供整个灵长类动物群体商业使用的恒河猴寡核苷酸芯片。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The technology of microarray analysis of gene expression is not yet being optimally applied to nonhuman primate research. One widely available tool for microarray analysis are the Affymetrix gene chips which can analyze over 15,000 mRNAs per chip. However, because the Affymetrix chips are based on short oligonucleotides for which a single base mismatch will prevent hybridization, it is generally thought that Affymetrix chips are not suitable for cross-species hybridization, even for species as close as monkeys and humans. In this project, which is a collaboration between the ONPRC and the University of Nebraska, construction of specific rhesus monkey microarray chips will be facilitated by generating rhesus cDNA sequences using targeted PCR techniques. To date, over 3,000 sequences have been generated and deposited to genbank. The next step will be to combine these sequences with other available rhesus DNA sequences to design rhesus oligonucleotide chips that will be commercially available to the entire primate community.