SNP DISCOVERY AND LD ANALYSIS IN RHESUS MACAQUES

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 该项目旨在识别300个猕猴基因中的单核苷酸多态(SNPs)，并利用这些SNPs来评估这些基因之间的连锁不平衡(LD)程度。具体地说，与非人类灵长类研究显著相关的300个基因将在24只无关的中国和印度猕猴身上进行分析。将对每个基因的三个不同区域(5‘、3’和一个中间区)进行PCR扩增、测序和比较，以识别序列变异。将对汇编的SNP数据进行分析，以确定印度和中国人群的等位基因频率。SNP等位基因也将被用来估计LD衰减率随物理距离的变化。结果将在公共数据库DBSNP和MonkeySNP中报告。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This project aims to identify single nucleotide polymorphisms (SNPs) in 300 rhesus macaque genes and to use those SNPs to assess the extent of linkage disequilibrium (LD) across the genes. Specifically, 300 genes with significant relevance to non-human primate research will be analyzed in 24 unrelated Chinese and Indian rhesus macaques. Three distinct regions per gene (5', 3' and one intermediate region) will be PCR amplified, sequenced and compared to identify sequence variants. The compiled SNP data will be analyzed to identify allele frequencies in both Indian and Chinese populations. The SNP alleles will also be used to estimate the rate of LD decay with physical distance. The results will be reported in the public databases dbSNP and MonkeySNP.