Establishment & maintenance of a closed CPRC SPF colony

描述(由申请人提供)： 这项提议的目标是在加勒比灵长类研究中心(CPRC)的Sabana Seca野外站维持一个封闭的无特定病原体(SPF)恒河猴供应和繁殖群体计划。具有基因特征的印度裔猴子的创始种群将由中国红十字会捐赠，其中将包括大约457只SPF恒河猴。来自这个封闭群体的动物将被安置在繁殖玉米床中，并将每季度接受四种目标病毒(疱疹B、STLV-1、SIV和SRV)的检测。将使用一组高度多态的遗传标记来描述群体特征并优化繁育群体的结构，最大限度地提高其遗传异质性水平。到第五年，该计划将产生总计833只SPF恒河猴后代，这将有助于满足对这些动物日益增长的需求。在这笔赠款结束时，预计该计划每年将生产约210只动物，从而实现自给自足。CPRC将继续为艾滋病和其他研究项目做出重大贡献，为NIH赞助的研究项目提供高质量和健康的SPF恒河猴。

DESCRIPTION (provided by applicant): The objective of this proposal is to maintain a closed specific pathogen free (SPF) rhesus macaque supply and breeding colony program at the Sabana Seca Field Station of the Caribbean Primate Research Center (CPRC). The founding stock of genetically characterized Indian-origin monkeys will be donated by CPRC and will consist of approximately 457 SPF rhesus macaques. Animals from this closed colony will be housed in reproduction corncribs, and will be quarterly tested for the presence of four target viruses (Herpes B, STLV-1, SIV and SRV). A panel of highly polymorphic genetic markers will be used to characterize the colony and to optimize the structure of breeding groups, maximizing the level of their genetic heterogeneity. By year five, the program will produce a total estimated offspring of 833 SPF rhesus macaques, which will help meet the increased demand for these animals. At the end of this grant, it is expected that this program will produce about 210 animals/year, thus attaining self-sufficiency. CPRC will continue to make significant contributions to AIDS and to other research programs by providing high quality and healthy SPF rhesus monkeys to NIH-sponsored research initiatives.