MAPP Research Networdk TIssue Analysis & Technology Core

描述(由申请人提供)：本申请的总体目标是建立一个组织分析和技术核心(TATC)，以支持RFA-DK-07-003中描述的慢性盆腔疼痛(MAPP)研究的多学科方法。MAPP研究网络将利用多地点和多学科的方法，为慢性盆腔疼痛综合征的发病机制和治疗提供新的见解，并为当前和未来的研究工作建立一个新的、有价值的生物库资源。作为对RFA的响应，TATC将与MAPP网络中的所有研究实体互动，并在以下领域提供专业知识和资源：1)MAPP发现站点产生的所有体液和组织标本的生物库收集、管理和分发，以及从这些标本生成专门的分析平台，如蛋白质和/或组织阵列、DMA或RNA提取，如发现站点工作或辅助项目计划所需；2)组织病理学和组织形态测量，包括诊断解释、石蜡加工和显微切割术、冷冻显微切割术、激光捕获显微切割、免疫组织化学和计算机辅助成像/图像分析；3)蛋白质组学/代谢组学，提供最先进的集中质谱学设施，以协助正确收集和处理样本，为蛋白质组和/或代谢组学研究提供咨询协助，并提供各种分析的性能，包括二维凝胶和层析蛋白质组图谱、蛋白质阵列、细胞因子阵列、多重ELISA、RT-PCR、红外成像、质谱学和基于核磁共振的靶向和非靶向代谢物图谱分析、药代动力学研究、氧化应激评估和质谱分析；以及4)基因组学/基因分型，提供咨询援助和最先进的基因组学设施，以协助进行先进的基因分型技术。我们的基本假设是，只有使用经过统一处理、处理、分析和解释的高质量、无偏见的患者标本，才能保证盆腔疼痛综合征生物学研究的质量、可靠性和适用性。 MAPP研究网络将是针对慢性盆腔疼痛综合征的同类研究中最大的倡议，慢性盆腔疼痛综合征是一种常见但未被充分研究的疾病。组织分析和技术核心设施的建立对这项研究计划的成功至关重要。

DESCRIPTION (provided by applicant): The overall aim of this application is to establish a Tissue Analysis and Technology Core (TATC) in support of the Multi-disciplinary Approach to the Study of Chronic Pelvic Pain (MAPP) Research Network as described in RFA-DK-07-003. The MAPP Research Network will utilize a multi-site and multi-disciplinary approach to provide new insights into the pathogenesis and treatment of Chronic Pelvic Pain Syndromes, and establish a new and valuable biorepository resource for current and future research efforts. In response to the RFA, the TATC will interact with all research entities in the MAPP Network and provide expertise and resources in the areas of: 1) Biorepository Collection, Management and Distribution for all body fluid and tissue specimens generated by the MAPP Discovery Sites, and the generation of specialized assay platforms from these specimens such as protein and/or tissue arrays, DMA or RNA extractions as needed for Discovery Site efforts or the Ancillary Projects Program; 2) Histopathology and Tissue Morphometry including diagnostic interpretation, paraffin processing and microtomy, cryomicrotomy, laser capture microdissection, immunohistochemistry, and computer-assisted imaging/ image analysis; 3) Proteomics/ Metabolomics by providing a state-of-the-art centralized mass spectrometry facility to assist with proper collection and handling of specimens, consultative assistance for proteomic and/or metabolomics studies, and performance of a wide variety of assays, including two-dimensional gel- and chromatography-based proteome profiling, protein arrays, cytokine arrays, multiplexed ELISA, RT-PCR, infrared imaging, mass spectrometry and NMR-based targeted and non-targeted metabolite profiling, pharmacokinetic studies, oxidative stress assessment, and mass spectrometry analyses; and 4) Genomics/ Genotyping by providing consultative assistance and a state-of-the-art genomics facility to assist with advanced genotyping techniques. It is our underlying hypothesis that the quality, reliability and applicability of biological research on pelvic pain syndromes can only be guaranteed through the use of high quality non-biased patient specimens that have been handled, processed, analyzed and interpreted in a uniform manner. The MAPP Research Network will be the largest research initiative of its kind for chronic pelvic pain syndrome, a common but understudied condition in men and women. The establishment of a Tissue Analysis and Technology Core Facility is crucial to the success of this research program.