CORE E

来自细菌中蛋白质生产核心的蛋白质用于晶体结构分析将是常规的 贴上Se-Met标签以直接启用阶段化。对于在其他宿主中产生的或最初由 合作者，阶段化的其他方法，例如通过多个同构的替换方法 下面描述的，将被使用。主要目标是(1)开发结晶筛和添加剂筛 专为蛋白质复合体配制，以优化获得衍射晶的可能性和(2) 为了使用高通量结晶方法来解决HIV辅助/调节蛋白的结构， 数据收集和结构确定。最终的目标是解决艾滋病毒的结构 在与细胞蛋白的复合体中的辅助/调节蛋白，并通过使用合理的 方法，如本提案通篇所述。

Proteins from the Protein Production Core produced in bacteria for crystal structure analysis will routinely be Se-Met labeled to directly enable phasing. For those produced in other hosts or initially provided by collaborators, additional approaches to phasing, such as via multiple isomorphous replacement methods as described below, will be used. The major objectives are (1) to develop crystallization and additive screens formulated specifically for protein complexes to optimize the probability of obtaining diffracting crystals and (2) to solve structures of HIV accessory/regulatory proteins using high-throughput approaches for crystallization, data collection, and structure determination. The ultimate goal is to solve the structures of HIV accessory/regulatory proteins in complexes with cellular proteins and to accomplish this by using rational approaches, as described throughout this proposal.