NUTRITIONAL AND CONTRACTILE REGULATION OF MUSCLE GROWTH

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 这个项目的重点是探索肌肉是如何生长的。肌肉萎缩是许多疾病的常见现象，如癌症、艾滋病、创伤、脓毒症、肾衰竭，在老年人中尤其普遍。具体目标是：(1)确定在一轮阻力运动之前或之后给予食物时，肌肉生长对摄食的短期反应的大小。(2)确定肌肉生长对食物的短期反应的大小，食物提供的能量与运动期间消耗的能量相当或超过。(3)确定女性和男性在举重运动前或之后进食时，单独举重和结合进食时肌肉生长的短期反应的差异。这些程序包括使用股导管、举重练习、采血和呼吸以及进行肌肉活组织检查。在每一次实验中，我们将不断地注入稳定的同位素。稳定的同位素没有放射性，可以安全地提供给人类。这些稳定的同位素使我们能够测量人类肌肉的短期生长。这些研究将提供对调节人类肌肉生长的机制的洞察，并将被用作开发干预措施的基础，以改善衰老、创伤、癌症和艾滋病等条件下的肌肉生长。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The focus of this project is to explore how muscles grow. Muscle wasting is a common phenomenon in many conditions, such as cancer, AIDS, trauma, sepsis, kidney failure, and is also particularly prevalent in the elderly. Specific aims are to: (1) determine the magnitude of the short-term response of muscle growth to feeding when the food is given before or after a bout of resistance exercise. (2) determine the magnitude of the short-term response of muscle growth to food that provides an amount of energy that either matches or exceeds the amount of energy used during the exercise. (3) determine the differences between women and men in the short-term response of muscle growth to weight lifting alone and in combination with feeding when ingested before or after a bout of weight lifting exercise. The procedures include the use of femoral catheters, weight lifting exercise, blood and breath collection and taking muscle biopsies. During each experiment we will be continuously infusing stable isotopes. Stable isotopes are not radioactive and can be given safely to humans. These stable isotopes allow us to be able to measure short-term muscle growth in humans. These studies will provide insight into the mechanisms that regulate human muscle growth, and will be utilized as a basis from which to develop interventions for improving muscle growth in conditions such as aging, trauma, cancer, and AIDS.