Component 4: Biochemical Core Loren Parsons

拟建的TSRI酒精研究中心的生化核心由两个部分组成 用于提供样品分析和化合物的组件(药理和 否则)到整个中心。核心的生化分析部分将提供 内源性大麻素的分析(Roberto/Siggins和Parsons成分，太妃糖试点项目)， 氨基酸(Roberto/Siggins和Parsons组分)、单胺(Rivier组分和 Taffe Pilot)、CRF(Roberto/Siggins组件)、NPY(Zorrilla/Weiss和Roberto/Siggins 成分)和神经活性类固醇(动物模型核心)。这些分析将在以下情况下执行 在活体脑微透析样本、死后脑组织和大鼠血浆中以及在 灵长类脑脊液的高灵敏度和高选择性方法，包括LC-MS，CE-LIF，HPLC-ED和 放射免疫分析技术。核心还将提供关于最佳样本收集的咨询， 处理和储存，并将指导涉及体内微透析技术的实验设计。 核心的化合物储存库部分将提供必要的标准 上述分析方法以及任何所需的内标。该组件还将 协调标准曲线的灵敏度和重现性的标准化 化验。必需的CRF、神经肽Y、内源性大麻素、氨基酸、单胺和 神经类固醇配体一旦可用，也将进行纯度分析。配位体 不能在商业上获得，将根据需要进行合成。

The Biochemical Core of the proposed TSRI Alcohol Research Center is comprised of two components designed to provide sample analyses and chemical compounds (pharmacological and otherwise) to the Center at large. The biochemical analysis component of the Core will provide analyses of endocannabinoids (Roberto/Siggins and Parsons components, Taffe pilot project), amino acids (Roberto/Siggins and Parsons components), monoamines (Rivier component and Taffe pilot), CRF (Roberto/Siggins component), NPY (Zorrilla/Weiss and Roberto/Siggins components) and neuroactive steroids (animal models core). These analyses will be performed on in vivo brain microdialysis samples, post-mortem brain tissue, and plasma from rats as well as in primate CSF using highly sensitive and selective methods including LC-MS, CE-LIF, HPLC-ED and RIA techniques. The Core will also provide consultation regarding optimum sample collection, handling and storage, and will guide experimental design involving in vivo microdialysis techniques. The chemical compound repository component of the Core will provide the necessary standards for the assays described above as well as for any required internal standards. This component will also coordinate the standardization of sensitivity and reproducibility of standard curves used in these assays. The required CRF, neuropeptide Y, endocannabinoid, amino acid, monoamine, and neurosteroid ligands will also be analyzed for purity as they become available. Ligands which cannot be obtained commercially will be synthesized as necessary.