ANTI-INFLAMMATORY PROPERTIES OF TRICLOSAN: TRICLOSAN ANTI-INFLAMMATORY (TAU))

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目及 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 本研究旨在证明三氯生可抑制口腔炎症和组织破坏。 本研究共招募12名受试者。受试者将仅参加一次研究访视。他们必须没有任何牙周炎的迹象才能参加研究。将通过静脉穿刺采集40 ml全血。为了研究炎症，各种实验将包括评价炎症反应，所述炎症反应将使用将被刺激以模拟人体内反应的人全血离体测定。 对于研究的体外部分，THP-1细胞将同时用 大肠杆菌LPS和三氯生。同时，牙龈成纤维细胞培养物将用大肠杆菌LPS和三氯生刺激。研究的两个部分都将使用1份牙膏、2份水和1份唾液的溶液测试三氯生，以确定三氯生对 细胞将在2、6和24小时时间点收集细胞裂解物。在2和6小时，将进行mRNA提取以提供一组早期和立即应答基因。24小时后，样本将 离心并用于炎性介质分析。将包括两个未用三氯生处理的对照样品。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The purpose of this study is to demonstrate that triclosan inhibits inflammation and tissue destruction of the oral cavity. A total of 12 participants will be recruited for the study. Subjects will attend only one study visit. They must not show any signs of periodontitis to participate in the study. Forty (40) ml of whole blood will be collected by venipuncture. In order to study inflammation, various experiments will consist of the evaluation of the inflammatory response that will be determined ex vivo using whole human blood that will be stimulated to mimic the human in vivo response. For the in vitro part of the study, the THP-1 cells will be stimulated simultaneously with Escherichia coli LPS and Triclosan. While in parallel, cultures of gingival fibroblast cells will be stimulated with Escherichia coli LPS and Triclosan. Both parts of the study will test triclosan using a solution of 1 part toothpaste, 2 parts water and 1 part saliva to determine the effect of triclosan on the cells. Cell lysates will be collected at 2, 6 and 24 hour time points. At 2 and 6 hours, mRNA extractions will be performed to provide a set of early and immediate response genes. At 24 hours the sample will be centrifuged and utilized for inflammatory mediator analyses. Two control samples not treated with Triclosan will be included.