Analytical Chemistry Core/George Rottinghaus

分析化学核心将为研究项目和植物/植物基因组学和营养/动物核心提供分析支持。该设施将最大限度地减少设备和人员成本，提供专家技术支持，培训和质量控制。该设施的具体目标是： (1)开发和/或验证用于定量活性药物化合物及其代谢物的分析程序;（2）为研究项目营养/动物核心制备的饲料的质量控制提供分析定量，并为植物/植物基因组学核心筛选植物样品;以及（3）测量研究项目研究受试者体液中活性药物化合物及其代谢物的循环水平。分析化学核心将评估和验证用于检测大豆中的植物雌激素、大蒜酸中的S-取代半胱氨酸衍生物、Lessertia frutescens（也称为Sutheriandia frutescens）中的sutheriandiosides和sutheriandins、罗布麻和胡黄连中的夹竹桃素以及接骨木中的酚类物质的分析方法。将对所有植物试验材料、商业化合物和饲料的纯度和/或活性或标记化合物进行常规检查。 将筛选接骨木、大豆和产夹竹桃素植物的活性成分，以确定用于研究的高效价和低效价材料。植物活性化合物或其代谢物的循环水平将为三个研究项目提供有价值的信息。

The Analytical Chemistry Core will provide analytical support for the Research Projects and the Botanical/Plant Genomics and Nutrition/Animal Cores. The facility will minimize equipment and personnel costs, provide expert technical support, training, and quality control. The specific aims ofthe facility are to: (1) develop and/or validate analytical procedures for quantifying active medicinal compounds and their metabolites; (2) provide analytical quantitation for quality control of diets prepared by the Nutrition/Animal Core for the Research Projects and screening of botanical samples for the Botanical/Plant Genomics Core; and (3) measure circulating levels of active medicinal compounds and their metabolites in body fluids of research subjects for the Research Projects. The Analytical Chemistry Core will evaluate and validate analytical methodology for testing phytoestrogens in soy, S-sustituted cysteine derivatives in gariic, sutheriandiosides and sutheriandins in Lessertia frutescens (also known as Sutheriandia frutescens), apocynin in Apocynum and Picrorhiza species, and the phenolics in elderberry. All botanical test materials, commercial compounds, and diets will be routinely examined for purity and/or active or marker compounds. Elderberry, soy and apocynin-producing botanicals will be screened for active constituents to identify high and low-potency materials for research studies. Circulating levels of botanical active compounds or their metabolites will provide valuable information for the three Research Projects.