STATISTICAL BASIS FOR DETERMINING SIGNIFICANCE OF LOCALIZATION OF MODIFICATIONS

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 蛋白质的翻译后修饰对于调节细胞过程至关重要，我们对这些过程的理解依赖于确定这些修饰的准确位置的能力。串联质谱仪可能非常适合于翻译后修饰位点的确定。已经提出了几种方法来在给定串联质谱图的情况下确定修饰的最可能位置，但目前没有根据统计显著性水平(即，偶然发生定位指派的风险)来判断定位指派的框架。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Post-translational modifications of proteins are critical for regulating cellular processes and our understanding of these processes relies on the ability to determine the precise location of these modifications. Tandem mass spectrometry is potentially well suited for the determination of post-translational modification sites. Several methods have been proposed to determine the most likely location for a modification given a tandem mass spectrum, but currently there is no framework for judging a localization assignment in terms of a statistical significance level  i.e., the risk that a localization assignment has occurred by chance.