Neuropathology and Tissue Culture Core

核心A：神经病理学和细胞培养的核心 神经病理学和细胞培养核心是该计划项目资助(PPG)的科学核心。这个 这个核心的核心作用是协助项目小组内三个研究项目的总体目标，以 确定参与艾滋病患者HIV脑炎发生发展的不同分子途径。 神经病理学和细胞培养核心将通过提供 来自被诊断为HIV脑炎的HIV-1感染者的手术和尸检脑组织材料，AS 以及没有中枢神经系统参与的HIV-1感染者的大脑和正常对照大脑。近距离 与项目1、2和3合作，我们将进行免疫组织化学分析，包括 病毒蛋白、细胞标记物和Rho/Rac途径不同下游分子的标记 以及它们与Pur-a(项目1)、TNFa和RAS途径的关系及其与IGF的关系- 1/IRS通路(方案2)、HIF-1通路及其与VPR的关系(方案3)。这个 神经病理学和细胞培养核心还将提供高度纯化的人胎儿小胶质细胞培养， 星形胶质细胞和按需神经元。将从大鼠和小鼠制备神经元的原代培养 海马体用于拟议的研究。已建立的细胞系，如PC12，将被维持、培养和 分发给项目。此外，来自HIV-1感染细胞的条件培养液将准备好供 个别项目。最后，核心将提供分化为不同细胞的神经球的培养 表型，包括神经元、少突胶质细胞和星形胶质细胞。拟议中的实验将有助于项目 1、2和3，以破译参与HIV脑炎发病机制的分子途径。

Core A: The Neuropathology and Cell Culture Core The Neuropathology and Cell Culture Core is the scientific core of this Program Project Grant (PPG). The central role of this Core is to assist the overall objectives of the three research projects within the PPG to determine the different molecular pathways involved in the development of HIV encephalitis in AIDS patients. The Neuropathology and Cell Culture Core will assist the participants of the Program Project by providing surgical and autopsy brain tissue material from HIV-1 infected patients diagnosed with HIV encephalitis, as well as brains from HIV-1 infected individuals with no CNS involvement and normal control brains. In close collaboration with Projects 1, 2 and 3, we will perform immunohistochemical analysis, including double labeling, of viral proteins, cellular markers, and the different downstream molecules of the Rho/Rac pathway and their association with Pur-a (Project 1), the TNFa and Ras pathway and its association with the IGF- 1/IRS pathway (Project 2), and the HIF-1 pathway and its association with Vpr (Project 3). The Neuropathology and Cell Culture Core will also provide highly purified cultures of human fetal microglia, astrocytes, and neurons on demand. Primary cultures of neurons will be prepared from rat and mouse hippocampus for the proposed studies. Established cell lines, such as PC12 will be maintained, cultured, and distributed to the projects. Also, Conditioned Media from HIV-1 infected cells will be prepared for use by the individual projects. Finally, the Core will provide cultures of neurospheres differentiated into different cellular phenotypes, including neurons, oligodendrocytes and astrocytes. The proposed experiments will aid Projects 1, 2 and 3 to decipher the molecular pathways involved in the pathogenesis of HIV encephalitis.