PRODUCTION OF MHC-DEFINED AND GENETICALLY IDENTICAL MODELS

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 目的：1)开发利用卵裂球分离创造基因完全相同的猴子的策略；2)利用辅助生殖技术繁殖由MHC定义的猴子家族。 对于人类疾病的生物医学研究来说，基因完全相同且由MHC定义的猴子将是极其有价值的模型。由于可供分配的合适雌性恒河猴数量有限，项目早期的进展受到阻碍。然而，在上次报告所述期间，随着赠款的免费延长，在改进技术方面取得了显著进展。在怀孕过程中，已经有几次成功的分裂胚胎移植。这项研究使用了WNPRC动物服务中心。 注：与爱滋病有关

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Objective: To 1) develop strategies for creating genetically identical monkeys using blastomere separation and 2) propagate a family of MHC-defined monkeys using assisted reproductive technologies. Genetically identical and MHC-defined monkeys would be extremely valuable models for biomedical studies of human disease. Progress early in the project was hampered by limited numbers of appropriate female rhesus monkeys for assignment. During the last reporting period, however, with a no cost extension of the grant, remarkable progress in the refinement of techniques was accomplished. There have been several successful transfers of split embryos with pregnancies in progress. This research used WNPRC Animal Services. Note: AIDS related