POST-EXPOSURE MONITORING OF LENTIVIRUS-INFECTED MACAQUES

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 分配到这个项目的动物已经成功地感染了灵长类动物慢病毒。其目的是在较长时间内继续进行临床、病毒学和免疫学监测。目前的动物已经感染HIV-1超过17年，并且所有动物都继续表现出对HIV-1蛋白的血清反应阳性。 这种持续但受控的HIV-1复制的迹象使我们设计了一个实验，通过用人源化小鼠单克隆抗CD 8抗体cM-T807治疗暂时耗尽体内CD 8+细胞来检查CD 8+细胞在病毒控制中的作用。 通常，CD 8-耗尽的病毒控制猕猴经历病毒复制的同时显著增加，然后当CD 8+细胞恢复到治疗前水平时重新获得控制。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Animals assigned to this project have been successfully infected with a primate lentivirus. The purpose is to continue clinical, virologic and immunologic monitoring for an extended period of time. The current animals have been infected with HIV-1 over 17 years at this time, and all continue to demonstrate seropositive responses to HIV-1 proteins. This indication of continuing but controlled HIV-1 replication has led us to design an experiment to examine the role of CD8+ cells in virus control by temporarily depleting CD8+ cells in vivo by treatment with the humanized mouse monoclonal anti-CD8 antibody, cM-T807. Generally, CD8-depleted virus-controlling macaques experience a coincident marked increase in virus replication and then regain control when CD8+ cells return to pretreatment levels.