COMPUTATIONAL IMAGING CORE

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 该核心设施提供光学显微镜、体视学、数字成像、图像数据库开发和咨询服务，主要为CNPRC提供服务，也为所有校园部门提供充值服务。我们的目标是帮助教师，员工和学生与他们的研究需求，定性和定量的应用。该核心协助制作出版质量的图像，并提供有关其设备使用的实验方法的咨询。 此外，它还从事数字化和校正来自各种来源的存档电影图像，但主要是CNPRC病理学服务创建的图像。 Core还提供注释服务，并将完全校正的数字图像上传到CNPRC图像数据库中。 在数据库中，标记的图像与特定动物的其他信息相链接，并提供给所有获准访问的人。 对于经过培训的CNPRC用户来说，堆芯中的仪器设备每周7天、每天24小时都可以使用。咨询，培训和支持服务，沿着与访问其他部门的设备，可从8-5周一至周五。 该设施容纳以下设备： * 用于冷冻切片的低温恒温器。 * 石蜡切片机和塑料切片机。 * 用于塑料加工和包埋的样品制备设备。 * 用于存档客户端、病理单元和核心数据的数据存储服务器（可扩展的多TB容量）。 这些系统提供了快速访问非常大的（多千兆字节）文件或数据集，否则需要几个小时才能从CNPRC的其他地点转移。 * 基于网络的仪器预订日历。 * 奥林巴斯BX 61研究显微镜，配备奥林巴斯MicroSuite图像分析软件和Intelligent Imaging Innovations SlideBook软件，用于体视学和图像数据收集。 * 第二台Olympus BX 61显微镜，配备Intelligent Imaging Innovations SlideBook软件，用于Core员工的体视学技术开发。 * 图形工作站、平板扫描仪和高质量彩色校正打印机，用于出版质量打印、将胶片转换为数字图像、图像校正和存档。 * 文档扫描仪用于快速数字化和OCR转换纸质记录。 * Delta Vision显微镜系统和计算机工作站，用于非常高分辨率的多维荧光成像和反卷积。 * 用于体视学数据离线分析的计算机工作站（由核心人员用于方法开发）和大型多维图像分析。 * 最近更新的Visiopharm维斯/CAST网格体视学系统，用于定量数据收集和自动图像分析。 * Photoshop、Illustrator、LightRoom和DreamWeaver软件，支持Core的所有数字成像工作  所有电脑均配备彩色平衡显示器。 * 一种荧光体视显微镜，配备有连接到计算机系统的高灵敏度摄像机。 * 双头明视野立体显微镜。 未来的补充： Core正在为Olympus BX 61显微镜系统之一购买非激光共聚焦附件，以提高荧光图像的质量。 核心希望购买一个宽视场变焦共焦显微镜系统的应用，需要一个非常广泛的放大倍率。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This Core facility provides light microscopy, stereology, digital imaging, image database development, and consultation services, primarily for the CNPRC, and also for all campus departments on a recharge basis. The goal is to assist faculty, staff, and students with their research needs for qualitative and quantitative applications. This Core assists with the production of publication quality images and offers consultation on experimental approaches for use with its equipment. Additionally, it is engaged in digitizing and correcting archived film images from various sources but primarily those created by the Pathology Service at the CNPRC. The Core also provides a service to annotate and upload fully corrected digital images into the CNPRC image database. Uploaded images are linked in the database to other information for a specific animal and made available to all who have been granted access. Instrumentation housed in the Core is available 24 hours a day, 7 days a week for trained CNPRC users. Consultation, training, and support service, along with access to the equipment for other departments, is available from 8-5 Monday through Friday. The facility houses the following equipment: * A Cryostat for frozen sections. * Microtomes for paraffin and plastic sectioning. * Sample preparation equipment for plastic processing and embedding. * Data storage servers (expandable multi-terabyte volumes) for archiving client, Pathology Unit and Core data. These systems provide rapid access to very large (multi-gigabyte) files or data sets that would otherwise require hours to transfer from other locations in the CNPRC. * Web-based calendar for instrument reservations. * An Olympus BX61 research microscope with Olympus MicroSuite image analysis software and Intelligent Imaging Innovations SlideBook software for stereology and image data collection. * A second Olympus BX61 microscope with Intelligent Imaging Innovations SlideBook software for stereology technique development by the Core's staff. * A graphics workstation, a flatbed scanner, and a high-quality color corrected printer for publication quality prints, converting film to digital images, image correction, and archiving. * Document scanners for rapid digitization and OCR conversion of paper records. * A Delta Vision Microscopy System and computer workstation for very high resolution multi-dimensional fluorescence imaging with deconvolution. * A computer workstation for offline analysis of stereology data (used for method development by the Core staff), and large multidimensional image analysis. * A recently updated Visiopharm VIS/CAST Grid Stereology System for quantitative data collection and automated image analysis. * Photoshop, Illustrator, LightRoom, and DreamWeaver software to support all digital imaging efforts of the Core  all computers are outfitted with spectrophotometrically color balanced monitors. * A fluorescence stereomicroscope outfitted with a high sensitivity camera coupled to a computer system. * A dual-head brightfield stereomicroscope. Future additions: The Core is in the process of purchasing a non-laser based confocal attachment for one of the Olympus BX61 microscope systems to improve the quality of fluorescent images. The Core hopes to purchase a widefield zoom confocal microscopy system for applications that require a very wide range of magnifications.