BIOMARKERS OF BRAIN PATHOLOGY

这个子项目是许多利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 该项目旨在开发一种使用液相色谱-质谱法的方案，以量化使用猴子模型滥用可卡因和使用小鼠模型实验诱导更年期导致的脑化学变化。 在本年度，谷氨酸和GABA的水平已被测量的微透析样本收集自猴子的纹状体自我管理可卡因。 在第二个项目中，在对照小鼠和过度表达淀粉样蛋白的小鼠中诱导卵巢衰竭，以模拟绝经期卵泡损失的特征。 分析将在未来一年进行，在神经病理学的发展，在控制与实验动物。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This project is designed to develop a protocols using liquid chromatography-mass spectroscopy to quantify changes in brain chemistry resulting from cocaine abuse using a monkey model and experimentally induced menopause using a mouse model. During the current year, levels of glutamate and GABA have been measured in microdialysis samples collected from the striatum of monkeys self-administering cocaine. In the second project, ovarian failure, to mimic the loss follicles characteristic of menopause, was induced in control mice and mice over-expressing the amyloid protein. Analyses will proceed in the coming year in the development of neuropathology in control versus experimental animals.