Flow Cytometry

项目总结（见说明）： 自1984年成立以来，CSHL FACS共享资源的目标一直是为癌症中心成员提供最先进的流式细胞仪设备和支持。该资源随着新兴技术和不断变化的研究需求而发展。1984年，主要用途是通过DNA含量或表达的细胞表面抗原分离细胞。荧光标记（如绿色）的出现 荧光蛋白GFP）在FACS应用中迎来了一个新阶段，因为癌症中心的成员使用GFP来跟踪转染的细胞，并从复杂的混合物中分离特定的细胞群。流式细胞仪分选速度和效率的提高使得以前不可能或不切实际的实验得以完成，例如分离大量肿瘤或干细胞进行基因组测序。 基因分析。最近推出的新一代蛋白质，荧光在各种颜色和毒性比他们的前辈，改变了流式细胞仪的要求，增加了对资源的需求。为了满足这些需求，FACS共享资源在位置、仪器和人员方面进行了一系列广泛的变化，旨在 扩大CSHL的流式细胞仪功能，提高癌症中心成员流式细胞仪的效率和可靠性。

PROJECT SUMMARY (See instructions): Since its inception in 1984, the goal of the CSHL FACS Shared Resource has been to give Cancer Center members access to state-of-the-art flow cytometry equipment and support. The Resource has evolved with emerging technologies and changing research needs. In 1984, the primary use was separation of cells by DNA content or by expressed cell-surface antigens. The advent of fluorescent markers (such as the green fluorescent protein GFP) ushered in a new phase in FACS usage, as Cancer Center members used GFP to track transfected cells, and to isolate particular populations of cells from complex mixtures. Increases in the speed and efficiency of sorting by FACS have enabled completion of experiments that once would have been impossible or impractical¿such as the isolation of large quantities of tumor or stem cells for genomic and genetic analyses. The recent introduction of the new generation of proteins that fluoresce in various colors and are less toxic than their predecessors, changed the requirements for flow cytometers and increased the demand for the Resource. In response to these demands, the FACS Shared Resource has undergone an extensive series of changes¿in location, instrumentation, and personnel¿designed to expand FACS capabilities at CSHL, and improve the efficiency and reliability of flow cytometry for Cancer Center members.