Proteomics

项目总结(见说明)： 蛋白质组学共享资源(PSR)是一个专门的技术设施，主要目标是蛋白质鉴定、表征和定量，并向CSHL的所有癌症中心成员开放。蛋白质组学共享资源位于贝克曼大楼一楼的W.M.凯克结构生物学实验室内。在过去的18个月中，CSHL完成了对 资源。一名在蛋白质组学方面具有重要专业知识的资深教员被招募来重新设计和监督共享资源。此外，还招募了一名在蛋白质组学和质谱学方面有经验的博士级科学家来管理和运营该设施。随着新工作人员的到位，购买了最先进的设备，并对实验室空间进行了广泛的翻新。该资源拥有最新、最先进的设备，包括一台Thermo LTQ-Orbitrap质谱仪、一台Thermo LTQ离子陷阱质谱仪、一台Agilent 6520四极飞行时间(QTOF)质谱仪和一台Thermo Vantage三重四极质谱仪，均配有纳米电喷雾(NanoESl)源。 第二台带有ETD源的Orbitrap Velos质谱计(由高端仪器计划资助)将于2010年底安装。另外，Accela高效液相色谱仪和自动进样器也可用于使用Vantage三元组的分析规模MRM工作。

PROJECT SUMMARY (See instructions): The Proteomics Shared Resource (PSR) is a specialized technical facility with the primary goal of protein identification, characterization and quantitation, and is open to all Cancer Center members at CSHL. The Proteomics Shared Resource is located within the W.M. Keck Structural Biology Laboratory on the ground floor ofthe Beckman building. In the last 18 months CSHL has completed a major restructuring ofthe Resource. A senior faculty member with significant expertise in proteomics was recruited to redesign and oversee the Shared Resource. In addition, a PhD-level scientist with experience in proteomics and mass spectrometry was recruited to manage and run the facility. With the new staff in place, state-of-the-art equipment was purchased and the laboratory space was extensively renovated. The Resource features new, state-of-the-art equipment, including: one Thermo LTQ-Orbitrap mass spectrometer, one Thermo LTQ iontrap mass spectrometer, one Agilent 6520 quadrupole time-of-flight (QTOF) mass spectrometer and one Thermo Vantage triple quadrupole mass spectrometer, all fitted with nano electrospray (NanoESl) sources. A second Orbitrap Velos mass spectrometer with ETD source (funded through the High-End Instrumentation program) is due to be installed in late 2010. An additional Accela HPLC and autosampler is also available for analytical scale MRM work using the Vantage triple-Quad.