The Ivis Spectrum CT for Pre-Clinical Oncology Research

描述（申请人提供）：请求提供资金，用于购买Ivis Spectrum CT光学和计算机断层扫描成像系统（Caliper Life Sciences，Hopkinton，MA）进行定量体内生物光子学分析。（即生物发光和荧光）成像，用于非侵入性和纵向监测（a）癌症和其它疾病过程，（B）癌症、免疫和干细胞的运输，（c）对治疗和其他干预的细胞反应，和（d）小动物（即小鼠和大鼠）肿瘤模型中的遗传途径表达。与我们现有的两个Ivis系统Ivis 100和200一样，这台最先进的仪器将安装在我们的小动物成像核心设施中，将成为纪念斯隆-凯特琳癌症中心（MSKCC）临床前和转化肿瘤学研究计划的关键、广泛使用的组成部分。体内生物发光和荧光成像已迅速成为世界范围内临床前生物医学研究的主要内容。然而，我们的Ivis 100和200系统的使用量非常大（现在已经超出了容量），而且它们的使用量还在继续增加-它们是迄今为止使用量最大的小动物成像仪器，支持整个MSKCC多达36个实验室。因此，没有备份/冗余。因此，当其中一个系统需要服务时，实验工作就会被延迟，甚至可能受到损害甚至损失。此外，Ivis 100和200现在分别使用了9年和6年，接近或已经超过其使用寿命，因此随时可能出现无法修复的故障。除了提供迫切需要的额外成像能力和备份外，Ivis光谱CT还将提供关键的新功能，包括（1）在光学和近红外（NIR）范围内的透射照明荧光成像，提供更好的光谱分辨率和荧光成像对比度;（2）三维（3D）/层析成像能力，提供关键的深度信息;以及（3）集成的基于CT的3D成像，以允许光学图像和解剖图像的无缝的接近完美的配准，从而在体内明确地定位光学信号病灶。我们的设施和中心拥有完善的技术和科学专业知识，可以在安装时充分和立即利用该仪器，以及有效的行政和财务基础设施，以支持和维护该仪器及其长期有效使用。

DESCRIPTION (provided by applicant): Funds are requested to purchase an Ivis Spectrum CT optical and computed tomography (CT) imaging system (Caliper Life Sciences, Hopkinton, MA) for quantitative in vivo bio photonic (i.e. bioluminescent and fluorescent) imaging for non-invasively and longitudinally monitoring (a) cancer and other disease processes, (b) trafficking of cancer, immune, and stem cells, (c) cellular responses to therapy and other interventions, and (d) genetic pathway expression in small-animal (i.e. mouse and rat) tumor models. Like our two existing Ivis systems, the Ivis 100 and 200, this state-of-the-art instrument, to be installed n our Small-Animal Imaging Core Facility, will be a critical, broadly used component of the pre-clinical and translational oncology research program at Memorial Sloan-Kettering Cancer Center (MSKCC). In vivo bioluminescent and fluorescent imaging has rapidly become a staple of pre-clinical biomedical research world-wide. However, both our Ivis 100 and 200 systems are used very heavily (and, now beyond capacity) and their use continues to increase - they are by far the most heavily used of our small-animal imaging instruments, supporting up to three dozen laboratories throughout MSKCC. Thus, there is no back-up/redundancy. When one of these systems requires service, therefore, experimental work is delayed and potentially compromised and even lost. Further, the Ivis 100 and 200 are now 9- and 6-years-old, respectively, and approaching or already beyond their useful lifespan and thus subject to irreparable failure at any moment. Besides providing urgently needed additional imaging capacity and back-up, the Ivis Spectrum CT would provide critical new functionality, including (1) trans-illumination fluorescence imaging in both the optical and near-infrared (NIR) range, providing far better spectral resolution and fluorescence-imaging contrast; (2) three-dimensional (3D)/ tomographic imaging capability to provide critical depth information; and (3) integrated CT-based 3D imaging to allow seamless near-perfect registration of optical and anatomic images and thereby unambiguous localization in vivo of optical signal foci. Our Facility and Center have well-established technical and scientific expertise to fully and immediately utilize this instrument upo its installation and an efficient administrative and financial infrastructure to support and maintan this instrument and its effective use long-term.